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Immunohistochemical staining of human colon shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human stomach shows strong granular cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Expression
RNA: detected in 9 tissues Protein: detected in 19 cell types
RNA: detected in 9 tissues Protein: detected in 66 cell types
RNA: detected in 9 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:600
1:3000
1:500
Literature conformity
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
Not consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030878
Antibody CAB024997
Antibody CAB025649
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403676)
Target mass (kDa)
14.7
14.7, 12.1
14.7, 12.1
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA030878
Antibody CAB024997
Antibody CAB025649
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.