Fields »
ABOUT
HELP
BLOG
CATSPER4

ANTIBODY INFORMATION
 
 

Antibody HPA045257

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045257  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 9  

References

  

Proper citation

 Atlas Antibodies Cat#HPA045257, RRID:AB_10968995  

VALIDATION SUMMARY
 
 

Antibody HPA045257

  
Immunohistochemistry

Supported  
Western blot

Uncertain  
Protein array

Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045257

  

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferus ducts.  

Expression

 RNA: detected in 1 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:1000  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045257

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 55.1, 54.1, 42.8  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA045257

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA045257

  

Antigen

 Recombinant protein fragment  

Length (aa)

 77  

Antigen sequence

 
GQQQRITFSETGAEEEEENDQLPLVHCVVARSEKSGLLQEPLAGGPLSNL
SENTCDNFCLVLEAIQENLRQYKEIRD
 

Matching transcripts

 CATSPER4-001 - ENSP00000390423 [100%]
CATSPER4-002 - ENSP00000341006 [100%]
CATSPER4-201 - ENSP00000477936 [100%]
 

Other gene match

  
ANTIGEN VIEW
CATSPER4-001
CATSPER4-002
CATSPER4-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.