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Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm.
Antibody dilution
1:47
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA034487
Antibody HPA057358
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in a subset of cells in exocrine pancreas.
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in decidual cells.
Expression
RNA: detected in 1 tissues Protein: detected in 9 cell types
RNA: detected in 1 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:3500
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA034487
Antibody HPA057358
Standard validation
Uncertain
No bands detected.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404938)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY404938)
Target mass (kDa)
35.9
35.9
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA034487
Antibody HPA057358
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
