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ANTIBODY INFORMATION
 
 

Antibody HPA048088

  

Antibody HPA055010

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA048088   HPA055010  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   14  

References

    

Proper citation

 Atlas Antibodies Cat#HPA048088, RRID:AB_2680259   Atlas Antibodies Cat#HPA055010, RRID:AB_2682670  

VALIDATION SUMMARY
 
 

Antibody HPA048088

  

Antibody HPA055010

  
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA048088

  

Antibody HPA055010

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 5 cell types		   RNA: detected in 37 tissues
Protein: detected in 56 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:100  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA048088

  

Antibody HPA055010

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 16   16, 13.5, 11.5, 11.1  

Antibody dilution

 1:120   1:300  

PROTEIN ARRAY
 
 

Antibody HPA048088

  

Antibody HPA055010

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:30000   1:2450  

ANTIGEN INFORMATION
 
 

Antibody HPA048088

  

Antibody HPA055010

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 29   31  

Antigen sequence

 
AAEGVLATRSDEPARDDAAVETAEEAKEP
  
AGLQPYLDQINVIEEQVAALEQAAYKLDAYS
 

Matching transcripts

 BLOC1S2-002 - ENSP00000359398 [100%]
  BLOC1S2-001 - ENSP00000410865 [100%]
BLOC1S2-002 - ENSP00000359398 [100%]
BLOC1S2-004 - ENSP00000464387 [100%]
BLOC1S2-201 - ENSP00000481847 [100%]
BLOC1S2-202 - ENSP00000484340 [100%]
 

Other gene match

    
ANTIGEN VIEW
BLOC1S2-001
BLOC1S2-002
BLOC1S2-004
BLOC1S2-201
BLOC1S2-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.