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MYBPC1

ANTIBODY INFORMATION
 
 

Antibody HPA021004

  

Antibody HPA027614

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA021004   HPA027614  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   6  

References

 2    

Proper citation

 Atlas Antibodies Cat#HPA021004, RRID:AB_1854232   Atlas Antibodies Cat#HPA027614, RRID:AB_10602711  

VALIDATION SUMMARY
 
 

Antibody HPA021004

  

Antibody HPA027614

  
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021004

  

Antibody HPA027614

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human skeletal muscle shows moderate cytoplasmic positivity in myocytes.  

Expression

 RNA: detected in 9 tissues
Protein: detected in 11 cell types		   RNA: detected in 9 tissues
Protein: detected in 1 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:100   1:50  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021004

  

Antibody HPA027614

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 131.7, 129.6, 129.1, 128.4, 128.3, 127.8, 127.6, 127.1, 126.5, 126.4, 88.1   131.7, 129.6, 129.1, 128.4, 128.3, 127.8, 127.6, 127.1, 126.5, 126.4, 115.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA021004

  

Antibody HPA027614

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA021004

  

Antibody HPA027614

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 134   142  

Antigen sequence

 
DWTLVETPPGEEQAKQNANSQLSILFIEKPQGGTVKVGEDITFIAKVKAE
DLLRKPTIKWFKGKWMDLASKAGKHLQLKETFERHSRVYTFEMQIIKAKD
NFAGNYRCEVTYKDKFDSCSFDLEVHESTGTTPN
  
YYSQPILVKEIIEPPKIRIPRHLKQTYIRRVGEAVNLVIPFQGKPRPELT
WKKDGAEIDKNQINIRNSETDTIIFIRKAERSHSGKYDLQVKVDKFVETA
SIDIQIIDRPGPPQIVKIEDVWGENVALTWTPPKDDGNAAIT
 

Matching transcripts

 MYBPC1-001 - ENSP00000400908 [100%]
MYBPC1-002 - ENSP00000447362 [100%]
MYBPC1-003 - ENSP00000376665 [100%]
MYBPC1-004 - ENSP00000448175 [100%]
MYBPC1-006 - ENSP00000354845 [100%]
MYBPC1-007 - ENSP00000447900 [100%]
MYBPC1-008 - ENSP00000440034 [100%]
MYBPC1-009 - ENSP00000354849 [100%]
MYBPC1-010 - ENSP00000446128 [100%]
MYBPC1-011 - ENSP00000442847 [100%]
MYBPC1-013 - ENSP00000447660 [100%]
MYBPC1-024 - ENSP00000449702 [100%]
MYBPC1-201 - ENSP00000388989 [100%]
  MYBPC1-001 - ENSP00000400908 [100%]
MYBPC1-002 - ENSP00000447362 [100%]
MYBPC1-003 - ENSP00000376665 [100%]
MYBPC1-004 - ENSP00000448175 [100%]
MYBPC1-006 - ENSP00000354845 [100%]
MYBPC1-007 - ENSP00000447900 [100%]
MYBPC1-008 - ENSP00000440034 [100%]
MYBPC1-009 - ENSP00000354849 [100%]
MYBPC1-010 - ENSP00000446128 [100%]
MYBPC1-011 - ENSP00000442847 [100%]
MYBPC1-012 - ENSP00000447116 [100%]
MYBPC1-013 - ENSP00000447660 [100%]
MYBPC1-024 - ENSP00000449702 [100%]
 

Other gene match

    
ANTIGEN VIEW
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MYBPC1-001
MYBPC1-002
MYBPC1-003
MYBPC1-004
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MYBPC1-007
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MYBPC1-009
MYBPC1-010
MYBPC1-011
MYBPC1-012
MYBPC1-013
MYBPC1-024
MYBPC1-201
»

RELEVANT PUBLICATIONS
 
 

Antibody HPA021004

  

Antibody HPA027614

  

1.

 Expression profiles of muscle disease-associated genes and their isoforms during differentiation of cultured human skeletal muscle cells
Abdul-Hussein S et al
BMC Musculoskelet Disord 2012;13:262
    

2.

 Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23
    
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.