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ANTIBODY INFORMATION VALIDATION SUMMARY IMMUNOCYTOCHEMISTRY IMMUNOHISTOCHEMISTRY WESTERN BLOT PROTEIN ARRAY ANTIGEN INFORMATION RELEVANT PUBLICATIONS

ANTIBODY INFORMATION

 
 

Antibody HPA071290

 

Provider

Atlas Antibodies
Sigma-Aldrich
 

Product name

HPA071290  

Host species

Rabbit  

Clonality

pAb  

Purity

Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 

Released in version

14  

References

 

Proper citation

Atlas Antibodies Cat#HPA071290, RRID:AB_2686381  

VALIDATION SUMMARY

 
 

Antibody HPA071290

 
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY

 
 

Antibody HPA071290

 

Standard validation

Approved  

 

Figure description

Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in subset of non-germinal center cells.  

Expression

RNA: detected in 14 tissues
Protein: detected in 6 cell types
 

Retrieval

HIER pH6  

Antibody dilution

1:20  

Literature conformity

Partly consistent with gene/protein characterization data.  

RNA consistency

Mainly not consistent with RNA expression data.  

WESTERN BLOT

 
 

Antibody HPA071290

 

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.
 

 

Figure description

 

Target mass (kDa)

59.8, 47.5  

Antibody dilution

1:80  

PROTEIN ARRAY

 
 

Antibody HPA071290

 

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.
 

 

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

1:500  

ANTIGEN INFORMATION

 
 

Antibody HPA071290

 

Antigen

Recombinant protein fragment  

Length (aa)

47  

Antigen sequence

KEEEGLATYWQSITWSRYPSPLEANITLSWNKTVELTDDVVMTFEYG
 

Matching transcripts

NTNG2-001 - ENSP00000376921 [100%]
NTNG2-201 - ENSP00000361252 [100%]
 

Other gene match

 
ANTIGEN VIEW
NTNG2-001
NTNG2-201
 

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