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Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA019536 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA018849 across 74 cell types.
Standard validation
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in reaction center cells and lymphoid cells outside reaction centra.
Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal and non germinal center cells.
Immunohistochemical staining of human tonsil shows moderate cytoplasmic positivity in germinal and non-germinal center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 23 cell types
RNA: detected in 37 tissues Protein: detected in 22 cell types
RNA: detected in 37 tissues Protein: detected in 63 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:2000
1:115
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018849
Antibody HPA019536
Antibody CAB033987
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414045)
Target mass (kDa)
44.8, 44.6, 41.6, 22
22.1
44.8, 44.6, 41.6, 31, 22.1, 22, 18.8, 13
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA018849
Antibody HPA019536
Antibody CAB033987
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.