Fields »
ABOUT
HELP
BLOG
ZNF418

ANTIBODY INFORMATION
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA043800   HPA053762   HPA072145  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 ZNF552 - ENSG00000178935 [80%]
ZNF814 - ENSG00000204514 [80%]
ZNF587B - ENSG00000269343 [84%]
   ZNF552 - ENSG00000178935 [100%]
ZNF814 - ENSG00000204514 [100%]
ZNF587B - ENSG00000269343 [100%]
   ZNF552 - ENSG00000178935 [96%]
ZNF814 - ENSG00000204514 [96%]
ZNF587B - ENSG00000269343 [100%]
  

Released in version

 12   12   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA043800, RRID:AB_2678675   Atlas Antibodies Cat#HPA053762, RRID:AB_2682252    

VALIDATION SUMMARY
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Approved   Approved    
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line HEK 293 shows localization to nucleus.  

Antibody dilution

     1:70  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  

Standard validation

 Approved   Approved    

     

Figure description

 Immunohistochemical staining of human placenta shows strong membranous positivity in placental trophoblastic cells.   Immunohistochemical staining of human skin shows cytoplasmic positivity in epidermal cells.    

Expression

 RNA: detected in 35 tissues
Protein: detected in 8 cell types		   RNA: detected in 35 tissues
Protein: detected in 37 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:300   1:225    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 97.3, 80.2, 77.9, 72.2, 68.5, 65.5, 49.2, 46.2, 45.5   97.3, 80.2, 77.9, 72.2, 68.5, 65.5, 49.2, 46.2, 45.5   97.3, 80.2, 77.9, 72.2, 68.5, 65.5, 49.2, 46.2, 45.5  

Antibody dilution

 1:250   1:130   1:230  

PROTEIN ARRAY
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1000   1:2800  

ANTIGEN INFORMATION
 
 

Antibody HPA043800

  

Antibody HPA053762

  

Antibody HPA072145

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 25   29   27  

Antigen sequence

 
SGLLLQEATHTGEKSNSKPECESPF
  
QGTHHKQKLHRCEAWGNKLYDSGNFHQHQ
  
PYRGSVEEALFVKRCKLHVSGESSVFS
 

Matching transcripts

 ZNF418-001 - ENSP00000379451 [100%]
ZNF418-002 - ENSP00000471652 [100%]
ZNF418-003 - ENSP00000407039 [100%]
ZNF418-004 - ENSP00000468968 [100%]
ZNF418-201 - ENSP00000483763 [100%]
  ZNF418-001 - ENSP00000379451 [87%]
ZNF418-002 - ENSP00000471652 [87%]
ZNF418-003 - ENSP00000407039 [87%]
ZNF418-004 - ENSP00000468968 [87%]
ZNF418-201 - ENSP00000483763 [87%]
  ZNF418-001 - ENSP00000379451 [81%]
ZNF418-002 - ENSP00000471652 [81%]
ZNF418-003 - ENSP00000407039 [81%]
ZNF418-004 - ENSP00000468968 [81%]
ZNF418-201 - ENSP00000483763 [81%]
 

Other gene match

 ZNF552 - ENSG00000178935 [80%]
ZNF814 - ENSG00000204514 [80%]
ZNF587B - ENSG00000269343 [84%]
   ZNF552 - ENSG00000178935 [100%]
ZNF814 - ENSG00000204514 [100%]
ZNF587B - ENSG00000269343 [100%]
   ZNF552 - ENSG00000178935 [96%]
ZNF814 - ENSG00000204514 [96%]
ZNF587B - ENSG00000269343 [100%]
  
ANTIGEN VIEW
ZNF418-001
ZNF418-002
ZNF418-003
ZNF418-004
ZNF418-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.