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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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C5AR1
  • TISSUE

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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

C5AR1

Gene description

Complement component 5a receptor 1

Protein class

CD markers
G-protein coupled receptors
Predicted intracellular proteins
Predicted membrane proteins

Predicted localization

Intracellular,Membrane

Number of transcripts

2
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Cell line enhanced (U-87 MG, U-937)
HPA (normal tissue):Tissue enhanced (appendix)

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in subsets of immune cells in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Approved based on 2 antibodies.
HPA014520 , CAB002720
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PROGNOSTIC SUMMARY

Prognostic marker in renal cancer (unfavourable), cervical cancer (favourable), testis cancer (unfavourable) and ovarian cancer (unfavourable).
Renal cancer p<0.001
Cervical cancer p<0.001
Testis cancer p<0.001
Ovarian cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA014520
CAB002720

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most malignant cells were negative. Rare cases of the female genital tract showed moderate cytoplasmic staining.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Colorectal cancers and several cases of malignant gliomas, ovarian, endometrial and pancreatic cancers showed moderate cytoplasmic staining with additional membranous positivity in several cases. Remaining malignant cells were weakly stained or negative.

GENE INFORMATION

Gene name

C5AR1 (HGNC Symbol)

Synonyms

C5A, C5AR, C5R1, CD88

Description

Complement component 5a receptor 1 (HGNC Symbol)

Entrez gene summary

Chromosome

19

Cytoband

q13.32

Chromosome location (bp)

47290023 - 47322066

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000197405 (version 83.38)

Entrez gene

728

UniProt

P21730 (UniProt - Evidence at protein level)

neXtProt

NX_P21730

Antibodypedia

C5AR1 antibodies

PROTEIN BROWSER

C5AR1-002
C5AR1-003

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

C5AR1-002
ENSP00000470613
ENST00000594787
M0QZK7 [Direct mapping]
C5a anaphylatoxin chemotactic receptor 1
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   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Show all
17 aa
1.9 kDa
No 0
C5AR1-003
ENSP00000347197
ENST00000355085
P21730 [Direct mapping]
C5a anaphylatoxin chemotactic receptor 1
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CD markers
G-protein coupled receptors
   GPCRs excl olfactory receptors
   Chemokines and chemotactic factors receptors
Predicted membrane proteins
   Prediction method-based
   Membrane proteins predicted by MDM
   MEMSAT3 predicted membrane proteins
   MEMSAT-SVM predicted membrane proteins
   Phobius predicted membrane proteins
   SCAMPI predicted membrane proteins
   SPOCTOPUS predicted membrane proteins
   THUMBUP predicted membrane proteins
   TMHMM predicted membrane proteins
   GPCRHMM predicted membrane proteins
   # TM segments-based
   7TM proteins predicted by MDM
Protein evidence (Kim et al 2014)
Show all
GO:0000187 [activation of MAPK activity]
GO:0001856 [complement component C5a binding]
GO:0002430 [complement receptor mediated signaling pathway]
GO:0004878 [complement component C5a receptor activity]
GO:0004930 [G-protein coupled receptor activity]
GO:0004944 [C5a anaphylatoxin receptor activity]
GO:0005829 [cytosol]
GO:0005886 [plasma membrane]
GO:0005887 [integral component of plasma membrane]
GO:0006915 [apoptotic process]
GO:0006935 [chemotaxis]
GO:0006954 [inflammatory response]
GO:0006955 [immune response]
GO:0006968 [cellular defense response]
GO:0007165 [signal transduction]
GO:0007186 [G-protein coupled receptor signaling pathway]
GO:0007202 [activation of phospholipase C activity]
GO:0007204 [positive regulation of cytosolic calcium ion concentration]
GO:0007606 [sensory perception of chemical stimulus]
GO:0009986 [cell surface]
GO:0010575 [positive regulation of vascular endothelial growth factor production]
GO:0010759 [positive regulation of macrophage chemotaxis]
GO:0016021 [integral component of membrane]
GO:0016023 [cytoplasmic membrane-bounded vesicle]
GO:0016323 [basolateral plasma membrane]
GO:0021534 [cell proliferation in hindbrain]
GO:0030593 [neutrophil chemotaxis]
GO:0031100 [organ regeneration]
GO:0032494 [response to peptidoglycan]
GO:0032496 [response to lipopolysaccharide]
GO:0038178 [complement component C5a signaling pathway]
GO:0042789 [mRNA transcription from RNA polymerase II promoter]
GO:0043524 [negative regulation of neuron apoptotic process]
GO:0045177 [apical part of cell]
GO:0045766 [positive regulation of angiogenesis]
GO:0050679 [positive regulation of epithelial cell proliferation]
GO:0050830 [defense response to Gram-positive bacterium]
GO:0070374 [positive regulation of ERK1 and ERK2 cascade]
GO:0090023 [positive regulation of neutrophil chemotaxis]
Show all
350 aa
39.3 kDa
No 7

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