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SPTAN1

ANTIBODY INFORMATION
 
 

Antibody HPA007927

  

Antibody CAB004581

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA007927   sc-46696  

Host species

 Rabbit   Mouse  

Clonality

 pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

    

Released in version

 4   2  

References

    

Proper citation

 Atlas Antibodies Cat#HPA007927, RRID:AB_1857435   Santa Cruz Biotechnology Cat#sc-46696, RRID:AB_671135  

VALIDATION SUMMARY
 
 

Antibody HPA007927

  

Antibody CAB004581

  
Immunocytochemistry

Supported    
Immunohistochemistry

Supported   Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA007927

  

Antibody CAB004581

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to microtubules & vesicles.    

Antibody dilution

 1:32    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA007927

  

Antibody CAB004581

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells and neuropil.   Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 60 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:50  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA007927

  

Antibody CAB004581

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 287.6, 285.1, 284.5, 282.8, 282.3   287.6, 285.1, 284.5, 282.8, 282.3, 18.4, 7.5  

Antibody dilution

 1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA007927

  

Antibody CAB004581

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA007927

  

Antibody CAB004581

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 126    

Antigen sequence

 
HQEFKSCLRSLGYDLPMVEEGEPDPEFEAILDTVDPNRDGHVSLQEYMAF
MISRETENVKSSEEIESAFRALSSEGKPYVTKEELYQNLTREQADYCVSH
MKPYVDGKGRELPTAFDYVEFTRSLF
  

											 
																				

																		

										
											
Matching transcripts

												
																					SPTAN1-004 - ENSP00000361816 [100%] 
SPTAN1-005 - ENSP00000361824 [100%] 
SPTAN1-016 - ENSP00000350882 [100%] 
SPTAN1-017 - ENSP00000486308 [100%] 
SPTAN1-019 - ENSP00000487444 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
SPTAN1-004
SPTAN1-005
SPTAN1-016
SPTAN1-017
SPTAN1-019
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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