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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & vesicles.
Antibody dilution
1:37
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA045634
Antibody HPA053812
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules .
Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 30 tissues Protein: detected in 36 cell types
RNA: detected in 30 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:35
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045634
Antibody HPA053812
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
116.3, 104.6
116.3, 104.6
Antibody dilution
1:250
1:190
PROTEIN ARRAY
Antibody HPA045634
Antibody HPA053812
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
