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MYO1C

ANTIBODY INFORMATION
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   SIGMA
  

Product name

 HPA001768   HPA005924   M3567  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 4   16   12  

References

 3      

Proper citation

 Atlas Antibodies Cat#HPA001768, RRID:AB_1854252   Atlas Antibodies Cat#HPA005924, RRID:AB_2667469   Sigma-Aldrich Cat#M3567, RRID:AB_477180  

VALIDATION SUMMARY
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  
Immunocytochemistry

 

Validated
    
Immunohistochemistry

Supported     Supported  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  

Formal validation: Tagged protein

  
Validated

Antibody staining overlaps with GFP tagged protein		    

     

Figure description

   Immunofluorescent staining of transgenic HeLa cells show antibody staining in plasma membrane and GFP expression in plasma membrane.    

Antibody dilution

   1:23    
 

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & plasma membrane.    

Antibody dilution

   1:23    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunohistochemical staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.     Immunohistochemical staining of human lung shows cytoplasmic and membranous positivity in pneumocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 72 cell types		     RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:75     1:350  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 121.7, 119.6, 119, 117.9   121.7, 119.6, 119, 117.9   121.7, 119.6, 119, 117.9, 30.7, 19.5, 19.2, 18.7, 15.9, 15.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001768

  

Antibody HPA005924

  

Antibody CAB037023

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 148   148    

Antigen sequence

 
GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDF
TEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEG
STLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV
  
GEEETLRRLGLERNPQSYLYLVKGQCAKVSSINDKSDWKVVRKALTVIDF
TEDEVEDLLSIVASVLHLGNIHFAANEESNAQVTTENQLKYLTRLLSVEG
STLREALTHRKIIAKGEELLSPLNLEQAAYARDALAKAVYSRTFTWLV
  

											 
																				

																		

										
											
Matching transcripts

												
																					MYO1C-001 - ENSP00000352834 [100%] 
MYO1C-002 - ENSP00000459174 [100%] 
MYO1C-003 - ENSP00000437685 [100%] 
MYO1C-010 - ENSP00000412197 [100%] 
MYO1C-201 - ENSP00000354283 [100%] 

											 
																						MYO1C-001 - ENSP00000352834 [100%] 
MYO1C-002 - ENSP00000459174 [100%] 
MYO1C-003 - ENSP00000437685 [100%] 
MYO1C-010 - ENSP00000412197 [100%] 
MYO1C-201 - ENSP00000354283 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
MYO1C-001
MYO1C-002
MYO1C-003
MYO1C-010
MYO1C-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA001768

												
										 
																				
											
											
											
Antibody HPA005924

												
										 
																				
											
											
											
Antibody CAB037023

												
										 
																		

																	

										
											
1.

												
																						The myosin motor Myo1c is required for VEGFR2 delivery to the cell surface and for angiogenic signaling

	Tiwari A et al

	Am J Physiol Heart Circ Physiol 2013;304(5):H687-96

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Analysis of an independent tumor suppressor locus telomeric to Tp53 suggested Inpp5k and Myo1c as novel tumor suppressor gene candidates in this region

	Hedberg Oldfors C et al

	BMC Genet 2015;16:80

			Application: WB				
											 
																						
											 
																						
											 
																				

																		

										
											
3.

												
																						Loss of functional MYO1C/myosin 1c, a motor protein involved in lipid raft trafficking, disrupts autophagosome-lysosome fusion

	Brandstaetter H et al

	Autophagy 2014;10(12):2310-23

			Application: WB				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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