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HIBCH

ANTIBODY INFORMATION
 
 

Antibody HPA036540

  

Antibody HPA036541

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036540   HPA036541  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036540, RRID:AB_10673355   Atlas Antibodies Cat#HPA036541, RRID:AB_2675182  

VALIDATION SUMMARY
 
 

Antibody HPA036540

  

Antibody HPA036541

  
Immunocytochemistry

Approved   Approved  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported  

Validated
  
Protein array

Approved   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036540

  

Antibody HPA036541

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:49   1:169  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036540

  

Antibody HPA036541

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA036541 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA036540 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA036541 across 73 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA036540 across 73 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.   Immunohistochemical staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		   RNA: detected in 37 tissues
Protein: detected in 65 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:900  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036540

  

Antibody HPA036541

  

Formal validation: Genetic

  
Validated

Downregulation visible in both siRNA lanes		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

   43.5, 42.8, 18.6  

Loading control

   Total protein image  

Antibody dilution

   1:376  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 43.5, 42.8, 38   43.5, 42.8, 18.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036540

  

Antibody HPA036541

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036540

  

Antibody HPA036541

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 96   97  

Antigen sequence

 
PRLQGKLGYFLALTGFRLKGRDVYRAGIATHFVDSEKLAMLEEDLLALKS
PSKENIASVLENYHTESKIDRDKSFILEEHMDKINSCFSANTVEEI
  
GSSFALEQLKVINKMSPTSLKITLRQLMEGSSKTLQEVLTMEYRLSQACM
RGHDFHEGVRAVLIDKDQSPKWKPADLKEVTEEDLNNHFKSLGSSDL
 

Matching transcripts

 HIBCH-001 - ENSP00000352706 [100%]
HIBCH-010 - ENSP00000376144 [100%]
HIBCH-201 - ENSP00000481055 [81%]
  HIBCH-001 - ENSP00000352706 [100%]
HIBCH-007 - ENSP00000386274 [100%]
HIBCH-201 - ENSP00000481055 [100%]
 

Other gene match

    
ANTIGEN VIEW
HIBCH-001
HIBCH-007
HIBCH-010
HIBCH-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.