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CEP290

ANTIBODY INFORMATION
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Abbot Laboratories
  

Product name

 HPA043918   HPA064397   N/A  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

      

Released in version

 12   13   6  

References

      

Proper citation

 Atlas Antibodies Cat#HPA043918, RRID:AB_2678730   Atlas Antibodies Cat#HPA064397, RRID:AB_2685255    

VALIDATION SUMMARY
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Approved     Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Approved   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.    

Antibody dilution

   1:200    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  

Standard validation

 Approved     Approved  

     

Figure description

 Immunohistochemical staining of human colon shows strong cytoplasmic positivity with a granular pattern in glandular cells.     Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in cytotrophoblasts.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 72 cell types		     RNA: detected in 35 tissues
Protein: detected in 55 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:1600     1:1200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 290.5, 290.4, 180.1, 100.1   290.5, 290.4, 100.1, 94.3   290.5, 290.4, 180.1, 100.1, 94.3, 19.4, 12.8  

Antibody dilution

 1:250   1:1420   1:500  

PROTEIN ARRAY
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:12000   1:17100    

ANTIGEN INFORMATION
 
 

Antibody HPA043918

  

Antibody HPA064397

  

Antibody CAB029995

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide  

Length (aa)

 100   103    

Antigen sequence

 
QVESINKELEITKEKLHTIEQAWEQETKLGNESSMDKAKKSITNSDIVSI
SKKITMLEMKELNERQRAEHCQKMYEHLRTSLKQMEERNFELETKFAELT
  
IIPSLERLVNAIESKNAEGIFDASLHLKAQVDQLTGRNEELRQELRESRK
EAINYSQQLAKANLKIDHLEKETSLLRQSEGSNVVFKGIDLPDGIAPSSA
SII
  

											 
																				

																		

										
											
Matching transcripts

												
																					CEP290-001 - ENSP00000448012 [100%] 
CEP290-005 - ENSP00000446905 [100%] 
CEP290-201 - ENSP00000308021 [100%] 
CEP290-003 - ENSP00000473863 [98%] 

											 
																						CEP290-001 - ENSP00000448012 [100%] 
CEP290-003 - ENSP00000473863 [100%] 
CEP290-201 - ENSP00000308021 [100%] 
CEP290-202 - ENSP00000380938 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CEP290-001
CEP290-003
CEP290-005
CEP290-201
CEP290-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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