ECI2 - Antibodies - The Human Protein Atlas

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ECI2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA022130

Antibody HPA031626

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA022130

HPA031626

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA022130, RRID:AB_1855176

Atlas Antibodies Cat#HPA031626, RRID:AB_2673965

VALIDATION SUMMARY

Antibody HPA022130

Antibody HPA031626

Immunocytochemistry

Supported

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA022130

Antibody HPA031626

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria & vesicles.

Immunofluorescent staining of human cell line Hep G2 shows localization to peroxisomes.

Antibody dilution

1:100

1:52

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA022130

Antibody HPA031626

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA031626 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA022130 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA031626 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA022130 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Expression

RNA: detected in 37 tissues
Protein: detected in 51 cell types

RNA: detected in 37 tissues
Protein: detected in 35 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:500

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA022130

Antibody HPA031626

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

43.6, 40.2

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA022130

Antibody HPA031626

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA022130

Antibody HPA031626

Antigen

Recombinant protein fragment

Length (aa)

109

124

Antigen sequence

SLPKEAARQNYVDLVSSLSPSLESSSQVEPGTDRKSTGFETLVVTSEDGI
TKIMFNRPKKKNAINTEMYHEIMRALKAASKDDSIITVLTGNGDYYSSGN
DLTNFTDIP

DRATFHTPFSHLGQSPEGCSSYTFPKIMSPAKATEMLIFGKKLTAGEACA
QGLVTEVFPDSTFQKEVWTRLKAFAKLPPNALRISKEVIRKREREKLHAV
NAEECNVLQGRWLSDECTNAVVNF

Matching transcripts

ECI2-001 - ENSP00000369468 [100%]
ECI2-003 - ENSP00000369461 [100%]
ECI2-005 - ENSP00000420309 [100%]
ECI2-006 - ENSP00000354737 [100%]

Other gene match

ANTIGEN VIEW

ECI2-001	ECI2-003	ECI2-005	ECI2-006

RELEVANT PUBLICATIONS

Antibody HPA022130

Antibody HPA031626

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells
Stadler C et al
Nat Methods 2013;10(4):315-23

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.