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ALPK2

ANTIBODY INFORMATION
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA027377   HPA027976   HPA028084   HPA029801  

Host species

 Rabbit   Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

        

Released in version

 6   6   6   6  

References

        

Proper citation

 Atlas Antibodies Cat#HPA027377, RRID:AB_10600635   Atlas Antibodies Cat#HPA027976, RRID:AB_10611246   Atlas Antibodies Cat#HPA028084, RRID:AB_10601996   Atlas Antibodies Cat#HPA029801, RRID:AB_10602006  

VALIDATION SUMMARY
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  
Immunocytochemistry

      Approved  
Immunohistochemistry

Uncertain   Uncertain   Uncertain   Uncertain  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  

Standard validation

       Approved  

       

Figure description

       Immunofluorescent staining of human cell line BJ shows localization to cytosol.  

Antibody dilution

       1:87  

Literature conformity

       The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  

Standard validation

 Uncertain   Uncertain   Uncertain   Uncertain  

       

Figure description

 Immunohistochemical staining of human duodenum shows moderate cytoplasmic and nuclear positivity in glandular cells.   Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells and decidual cells.   Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 11 tissues
Protein: detected in 57 cell types		   RNA: detected in 11 tissues
Protein: detected in 57 cell types		   RNA: detected in 11 tissues
Protein: detected in 63 cell types		   RNA: detected in 11 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:60   1:75   1:40  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.   Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 237   237   237   237  

Antibody dilution

 1:250   1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:500   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA027377

  

Antibody HPA027976

  

Antibody HPA028084

  

Antibody HPA029801

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 83   89   97   89  

Antigen sequence

 
SPLSSCLPIMTHASLGVDTHNSTGQIHDVPENDIVEPRKRQYVFPVSQKR
GTIENERGKPLPSSPDLTRFPCTSSPEGNVTDF
  
SSVDPVDEKELSVTDSLSAASETGGKENVNNVSQDQEEKQLKMDHTAFFK
KFLTCPKILESSVDPIDEISVIEYTRAGKPEPSETTPQG
  
EKPTTLTANNECFQATRETEDTSTVTIATEVHPAKYLAVSIPEDKHAGGT
EERFPRASHEKVSQFPSQVQVDHILSGATIKSTKELLCRAPSVPGVP
  
EEAPFTGTTTISFSNLGGVHKENASLAQHSEVKPCTCGPQHEEKQDRDGN
IPDNFREDLKYEQSISEANDETMSPGVFSRHLPKDARAD
 

Matching transcripts

 ALPK2-001 - ENSP00000354991 [100%]
  ALPK2-001 - ENSP00000354991 [100%]
  ALPK2-001 - ENSP00000354991 [99%]
  ALPK2-001 - ENSP00000354991 [99%]
 

Other gene match

        
ANTIGEN VIEW
ALPK2-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.