Expression of MAP3K3 in cancer - Summary

GENERAL INFORMATION
Gene name	MAP3K3
Gene description	Mitogen-activated protein kinase kinase kinase 3
Protein class	Cancer-related genes Enzymes Plasma proteins Predicted intracellular proteins
Predicted localization	Intracellular
Number of transcripts	5
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Expressed in all
HPA (normal tissue):	Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic expression in lung macrophages, a subset of cells in lymphoid tissues, heart and smooth muscle.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Pending external verification.
Reliability score	Uncertain based on 2 antibodies. HPA035411 , CAB007764
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PROGNOSTIC SUMMARY
Prognostic marker in endometrial cancer (unfavourable).
Endometrial cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA035411	CAB007764

GENE INFORMATION
Gene name	MAP3K3 (HGNC Symbol)
Synonyms	MAPKKK3, MEKK3
Description	Mitogen-activated protein kinase kinase kinase 3 (HGNC Symbol)
Entrez gene summary	This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Chromosome	17
Cytoband	q23.3
Chromosome location (bp)	63622415 - 63696303
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000198909 (version 83.38)
Entrez gene	4215
UniProt	Q99759 (UniProt - Evidence at protein level)
neXtProt	NX_Q99759
Antibodypedia	MAP3K3 antibodies

PROTEIN BROWSER

MAP3K3-001	MAP3K3-002	MAP3K3-004	MAP3K3-005	MAP3K3-007

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
MAP3K3-001 ENSP00000354927 ENST00000361357	Q99759 [Direct mapping] Mitogen-activated protein kinase kinase kinase 3 Show all	Enzymes ENZYME proteins Transferases Kinases STE Ser/Thr protein kinases MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000165 [MAPK cascade] GO:0000186 [activation of MAPKK activity] GO:0000187 [activation of MAPK activity] GO:0001568 [blood vessel development] GO:0004672 [protein kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0004709 [MAP kinase kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005622 [intracellular] GO:0005829 [cytosol] GO:0006468 [protein phosphorylation] GO:0035556 [intracellular signal transduction] GO:0043123 [positive regulation of I-kappaB kinase/NF-kappaB signaling] GO:0046777 [protein autophosphorylation] GO:0046872 [metal ion binding] GO:1900745 [positive regulation of p38MAPK cascade] Show all	657 aa 74.1 kDa	No	0
MAP3K3-002 ENSP00000464130 ENST00000584573	J3QRB6 [Direct mapping] Mitogen-activated protein kinase kinase kinase 3 Show all	MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000165 [MAPK cascade] GO:0000187 [activation of MAPK activity] GO:0004672 [protein kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005622 [intracellular] GO:0006468 [protein phosphorylation] Show all	653 aa 73.7 kDa	No	0
MAP3K3-004 ENSP00000354485 ENST00000361733	Q99759 [Direct mapping] Mitogen-activated protein kinase kinase kinase 3 Show all	Enzymes ENZYME proteins Transferases Kinases STE Ser/Thr protein kinases MEMSAT-SVM predicted membrane proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0000165 [MAPK cascade] GO:0000186 [activation of MAPKK activity] GO:0000187 [activation of MAPK activity] GO:0004672 [protein kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0004709 [MAP kinase kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005622 [intracellular] GO:0005829 [cytosol] GO:0006468 [protein phosphorylation] GO:0035556 [intracellular signal transduction] GO:0043123 [positive regulation of I-kappaB kinase/NF-kappaB signaling] GO:0046777 [protein autophosphorylation] GO:0046872 [metal ion binding] Show all	626 aa 70.9 kDa	No	0
MAP3K3-005 ENSP00000462086 ENST00000577395	J3KRN4 [Direct mapping] Mitogen-activated protein kinase kinase kinase 3 Show all	MEMSAT-SVM predicted membrane proteins Predicted intracellular proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000165 [MAPK cascade] GO:0000187 [activation of MAPK activity] GO:0004672 [protein kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005622 [intracellular] GO:0006468 [protein phosphorylation] Show all	622 aa 70.5 kDa	No	0
MAP3K3-007 ENSP00000461988 ENST00000579585	Q99759 [Direct mapping] Mitogen-activated protein kinase kinase kinase 3 Show all	Enzymes ENZYME proteins Transferases Kinases STE Ser/Thr protein kinases MEMSAT-SVM predicted membrane proteins SCAMPI predicted membrane proteins Predicted intracellular proteins Plasma proteins Cancer-related genes Mutational cancer driver genes Protein evidence (Ezkurdia et al 2014) Show all	GO:0000165 [MAPK cascade] GO:0000186 [activation of MAPKK activity] GO:0000187 [activation of MAPK activity] GO:0004672 [protein kinase activity] GO:0004708 [MAP kinase kinase activity] GO:0004709 [MAP kinase kinase kinase activity] GO:0005515 [protein binding] GO:0005524 [ATP binding] GO:0005622 [intracellular] GO:0005829 [cytosol] GO:0006468 [protein phosphorylation] GO:0035556 [intracellular signal transduction] GO:0043123 [positive regulation of I-kappaB kinase/NF-kappaB signaling] GO:0046777 [protein autophosphorylation] GO:0046872 [metal ion binding] Show all	657 aa 74.1 kDa	No	0