DMD - Antibodies - The Human Protein Atlas

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DMD

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA002725

Antibody HPA023885

Antibody CAB000119

Provider

Atlas Antibodies
Sigma-Aldrich

Lab Vision/NeoMarkers

Product name

HPA002725

HPA023885

MS-486

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA002725, RRID:AB_1078714

Atlas Antibodies Cat#HPA023885, RRID:AB_1847931

VALIDATION SUMMARY

Antibody HPA002725

Antibody HPA023885

Antibody CAB000119

Immunohistochemistry

Supported

Western blot

Uncertain

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA002725

Antibody HPA023885

Antibody CAB000119

Standard validation

Supported

Figure description

Immunohistochemical staining of human heart muscle shows strong cytoplasmic and membranous positivity in myocytes.

Immunohistochemical staining of human heart muscle shows distinct membranous positivity.

Immunohistochemical staining of human heart muscle shows moderate membranous positivity in myocytes.

Expression

RNA: detected in 36 tissues
Protein: detected in 62 cell types

RNA: detected in 36 tissues
Protein: detected in 26 cell types

RNA: detected in 36 tissues
Protein: detected in 2 cell types

Retrieval

HIER pH6

HIER pH9

Antibody dilution

1:25

1:3000

1:100

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA002725

Antibody HPA023885

Antibody CAB000119

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Target too small/large to be analyzed with the present setup.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

426.8, 426.1, 426, 88.8

426.8, 426.1, 426, 159.4, 143.2, 141.8, 141.4, 129, 110

426.8, 426.1, 426, 159.4, 143.2, 141.8, 141.4, 129, 110, 88.8, 72.2, 70.7, 70.4, 59.8, 27.4, 16.1, 14.5, 12, 5.3

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA002725

Antibody HPA023885

Antibody CAB000119

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2000

1:20000

ANTIGEN INFORMATION

Antibody HPA002725

Antibody HPA023885

Antibody CAB000119

Antigen

Recombinant protein fragment

Not known

Length (aa)

148

150

Antigen sequence

VVCQQSATQRLEHAFNIARYQLGIEKLLDPEDVDTTYPDKKSILMYITSL
FQVLPQQVSIEAIQEVEMLPRPPKVTKEEHFQLHHQMHYSQQITVSLAQG
YERTSSPKPRFKSYAYTQAAYVTTSDPTRSPFPSQHLEAPEDKSFGSS

KQNDVHRAFKRELKTKEPVIMSTLETVRIFLTEQPLEGLEKLYQEPRELP
PEERAQNVTRLLRKQAEEVNTEWEKLNLHSADWQRKIDETLERLQELQEA
TDELDLKLRQAEVIKGSWQPVGDLLIDSLQDHLEKVKALRGEIAPLKENV

Matching transcripts

DMD-001 - ENSP00000354923 [100%]
DMD-002 - ENSP00000288447 [100%]
DMD-006 - ENSP00000367948 [100%]
DMD-205 - ENSP00000479270 [100%]
DMD-206 - ENSP00000478150 [100%]