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TCEAL5

ANTIBODY INFORMATION
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA045564   HPA058982   HPA072199  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 TCEAL3 - ENSG00000196507 [100%]
TCEAL6 - ENSG00000204071 [100%]
   TCEAL3 - ENSG00000196507 [100%]
TCEAL6 - ENSG00000204071 [92%]
    

Released in version

 10   13   16  

References

      

Proper citation

 Atlas Antibodies Cat#HPA045564, RRID:AB_2679369   Atlas Antibodies Cat#HPA058982, RRID:AB_2683870    

VALIDATION SUMMARY
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Uncertain   Uncertain    
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.  

Antibody dilution

     1:8  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  

Standard validation

 Uncertain   Uncertain    

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells of seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferus ducts.    

Expression

 RNA: detected in 16 tissues
Protein: detected in 24 cell types		   RNA: detected in 16 tissues
Protein: detected in 10 cell types		    

Retrieval

 HIER pH6   HIER pH6    

Antibody dilution

 1:300   1:80    

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.   Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 23.3, 22.5, 22.2, 20.3   23.3, 22.5, 22.2, 20.3   23.3  

Antibody dilution

 1:250   1:80   1:80  

PROTEIN ARRAY
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA045564

  

Antibody HPA058982

  

Antibody HPA072199

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 34   25   22  

Antigen sequence

 
PKDSQEDLQERHLSSEEMMRECGDVSRAQEELRK
  
EGEPGDEGQLEDEGSQEKQGRSEGE
  
KLYKENEGKPENERNLESEGKP
 

Matching transcripts

 TCEAL5-001 - ENSP00000361765 [100%]
  TCEAL5-001 - ENSP00000361765 [92%]
  TCEAL5-001 - ENSP00000361765 [100%]
 

Other gene match

 TCEAL3 - ENSG00000196507 [100%]
TCEAL6 - ENSG00000204071 [100%]
   TCEAL3 - ENSG00000196507 [100%]
TCEAL6 - ENSG00000204071 [92%]
    
ANTIGEN VIEW
TCEAL5-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.