We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA050327 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA046995 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA050327 across 74 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA046995 across 74 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human rectum shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human rectum shows moderate cytoplasmic and nuclear positivity in glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 66 cell types
RNA: detected in 35 tissues Protein: detected in 55 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:650
1:900
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA046995
Antibody HPA050327
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
30.4, 29.8, 27.9
30.4, 29.8, 27.9
Antibody dilution
1:150
1:140
PROTEIN ARRAY
Antibody HPA046995
Antibody HPA050327
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
