EHMT2 - Antibodies - The Human Protein Atlas

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EHMT2

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA050550

Antibody HPA060259

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA050550

HPA060259

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA050550, RRID:AB_2681170

Atlas Antibodies Cat#HPA060259, RRID:AB_2684234

VALIDATION SUMMARY

Antibody HPA050550

Antibody HPA060259

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA050550

Antibody HPA060259

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nuclear speckles.

Antibody dilution

1:42

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA050550

Antibody HPA060259

Standard validation

Supported

Figure description

Immunohistochemical staining of human esophagus shows moderate nuclear positivity in squamous epithelial cells.

Expression

RNA: detected in 23 tissues
Protein: detected in 41 cell types

Retrieval

HIER pH6

Antibody dilution

1:120

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA050550

Antibody HPA060259

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

138.8, 135.4, 132.4, 129

Antibody dilution

1:500

1:140

PROTEIN ARRAY

Antibody HPA050550

Antibody HPA060259

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:7700

1:1650

ANTIGEN INFORMATION

Antibody HPA050550

Antibody HPA060259

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

TAAPAPPPLSQDVPGRADTSQPSARMRGHGEPRRPPCDPLADTIDSSGPS
LTLPNGGCLSAVGLPLGPGREALEKALVIQESERRKKLR

PRSEETLPKATPDSLEPAGPSSPASVTVTVGDEGADTPVGATPLIGDESE
NLEGDGDLRGGRILLGHATKSFPSS

Matching transcripts

EHMT2-001 - ENSP00000364687 [100%]
EHMT2-007 - ENSP00000364680 [100%]
EHMT2-008 - ENSP00000379078 [100%]
EHMT2-009 - ENSP00000364678 [100%]

Other gene match

ANTIGEN VIEW

EHMT2-001	EHMT2-007	EHMT2-008	EHMT2-009

RELEVANT PUBLICATIONS

Antibody HPA050550

Antibody HPA060259

Alternative splicing regulates the expression of G9A and SUV39H2 methyltransferases, and dramatically changes SUV39H2 functions
Mauger O et al
Nucleic Acids Res 2015;43(3):1869-82

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.