PSMB10 - Antibodies - The Human Protein Atlas

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PSMB10

TISSUE

CELL

PATHOLOGY

ANTIBODY INFORMATION

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA030224

HPA030225

sc-133236

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

Proper citation

Atlas Antibodies Cat#HPA030224, RRID:AB_2673382

Atlas Antibodies Cat#HPA030225, RRID:AB_10603231

Santa Cruz Biotechnology Cat#sc-133236, RRID:AB_2171876

VALIDATION SUMMARY

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Immunocytochemistry

Approved

Immunohistochemistry

Western blot

Supported

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A549 shows localization to cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Antibody dilution

1:48

1:200

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA030225 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA030224 across 46 cell lines.

Standard validation

Supported

Figure description

Immunohistochemical staining of human tonsil shows nuclear positivity in non germinal center.

Immunohistochemical staining of human lymph node shows distinct cytoplasmic and nuclear positivity in lymphoid cells.

Immunohistochemical staining of human spleen shows strong cytoplasmic and nuclear positivity in cells in white pulp and cells in red pulp.

Expression

RNA: detected in 37 tissues
Protein: detected in 29 cell types

RNA: detected in 37 tissues
Protein: detected in 43 cell types

RNA: detected in 37 tissues
Protein: detected in 40 cell types

Retrieval

HIER pH6

Antibody dilution

1:125

1:4000

1:250

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

28.9, 14.2

28.9

28.9, 14.2

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA030224

Antibody HPA030225

Antibody CAB034384

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

FRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAV
LEDRFQPNMTLEAAQGLLVEAVT

AGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTA
VLTQTVKPLTLELVEETVQAMEVE

Matching transcripts

PSMB10-001 - ENSP00000351314 [100%]
PSMB10-004 - ENSP00000463811 [100%]

PSMB10-001 - ENSP00000351314 [100%]

Other gene match

ANTIGEN VIEW

PSMB10-001	PSMB10-004

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.