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Immunohistochemical staining of human colon shows distinct staining in red blood cells.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in subset of bone marrow poietic cells and red blood cells were moderately stained.
Expression
RNA: detected in 37 tissues Protein: detected in 41 cell types
RNA: detected in 37 tissues Protein: detected in 27 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:700
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043780
Antibody CAB032534
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
15.3
15.3, 11.9
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA043780
Antibody CAB032534
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.