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ATP10A

ANTIBODY INFORMATION
 
 

Antibody HPA041496

  

Antibody HPA042509

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA041496   HPA042509  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 11   11  

References

    

Proper citation

 Atlas Antibodies Cat#HPA041496, RRID:AB_2677513   Atlas Antibodies Cat#HPA042509, RRID:AB_2678032  

VALIDATION SUMMARY
 
 

Antibody HPA041496

  

Antibody HPA042509

  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA041496

  

Antibody HPA042509

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in tubules.   Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cells in glomeruli.  

Expression

 RNA: detected in 29 tissues
Protein: detected in 62 cell types		   RNA: detected in 29 tissues
Protein: detected in 23 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:75   1:150  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA041496

  

Antibody HPA042509

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

    

Target mass (kDa)

 167.7   167.7  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA041496

  

Antibody HPA042509

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA041496

  

Antibody HPA042509

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 86   98  

Antigen sequence

 
PTQLQLARQLTRKSPRRCSAPKETFAQGRLPKDSGTEHSSGRTVKTSVPL
SQPSWHTQQPVCSLEASGEPSTVDMSMPVREHTLLE
  
DSEEEEVVPRGGSVSQRGSIGSHQSVRVVHRTQSTKSHRRTGSRAEAKRA
SMLSKHTAFSSPMEKDITPDPKLLEKVSECDKSLAVARHQEHLLAHLS
 

Matching transcripts

 ATP10A-001 - ENSP00000349325 [100%]
  ATP10A-001 - ENSP00000349325 [100%]
 

Other gene match

    
ANTIGEN VIEW
ATP10A-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.