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ANTIBODY INFORMATION
 
 

Antibody HPA053749

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053749  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 11  

References

  

Proper citation

 Atlas Antibodies Cat#HPA053749, RRID:AB_2682248  

VALIDATION SUMMARY
 
 

Antibody HPA053749

  
Immunohistochemistry

Uncertain  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053749

  

Standard validation

 Uncertain  

 

Figure description

 Immunohistochemical staining of human nasopharynx shows strong cytoplasmic positivity in subset of respiratory epithelial cells.  

Expression

 RNA: detected in 3 tissues
Protein: detected in 50 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:450  

Literature conformity

 No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA053749

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 61.2, 56.6  

Antibody dilution

 1:670  

PROTEIN ARRAY
 
 

Antibody HPA053749

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:5400  

ANTIGEN INFORMATION
 
 

Antibody HPA053749

  

Antigen

 Recombinant protein fragment  

Length (aa)

 82  

Antigen sequence

 
EPFDVSADETVEVVKLMIKDYFHIPLSEDKQGRRYLELMYAGAALKDSWS
LADVGISFCSTLKCFVKEEDKPTLYVFNAVTQ
 

Matching transcripts

 ANKUB1-001 - ENSP00000417635 [100%]
ANKUB1-201 - ENSP00000387907 [100%]
 

Other gene match

  
ANTIGEN VIEW
ANKUB1-001
ANKUB1-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.