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Field

Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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NRAS
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PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

NRAS

Gene description

Neuroblastoma RAS viral (v-ras) oncogene homolog

Protein class

Cancer-related genes
Disease related genes
Predicted intracellular proteins
RAS pathway related proteins

Predicted localization

Intracellular

Number of transcripts

1
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

Cytoplasmic and membranous expression in several tissues.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly not consistent with RNA expression data. Caution, targets protein from more than one gene.

Reliability score

Uncertain based on 2 antibodies.
HPA049830 , CAB010157
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PROGNOSTIC SUMMARY

Prognostic marker in liver cancer (unfavourable), pancreatic cancer (unfavourable) and renal cancer (unfavourable).
Liver cancer p<0.001
Pancreatic cancer p<0.001
Renal cancer p<0.001
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RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA049830
CAB010157

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong cytoplasmic and membranous immunoreactivity was displayed in several Hodgkin's lymphomas, colorectal and stomach cancers along with a few ovarian, endometrial, prostate, liver and pancreatic cancers. Remaining cancer cells were weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Cancer cells showed moderate cytoplasmic immunoreactivity. The majority of colorectal cancers and several lymphomas, pancreatic and endometrial cancers were strongly stained.

GENE INFORMATION

Gene name

NRAS (HGNC Symbol)

Synonyms

N-ras

Description

Neuroblastoma RAS viral (v-ras) oncogene homolog (HGNC Symbol)

Entrez gene summary

This is an N-ras oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. The encoded protein, which has intrinsic GTPase activity, is activated by a guanine nucleotide-exchange factor and inactivated by a GTPase activating protein. Mutations in this gene have been associated with somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia. [provided by RefSeq, Jun 2011]

Chromosome

1

Cytoband

p13.2

Chromosome location (bp)

114704469 - 114716894

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000213281 (version 83.38)

Entrez gene

4893

UniProt

P01111 (UniProt - Evidence at protein level)

neXtProt

NX_P01111

Antibodypedia

NRAS antibodies

PROTEIN BROWSER

NRAS-001

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

NRAS-001
ENSP00000358548
ENST00000369535
P01111 [Direct mapping]
GTPase NRas
Q5U091 [Target identity:100%; Query identity:100%]
Neuroblastoma RAS viral (V-ras) oncogene homolog; Neuroblastoma RAS viral (V-ras) oncogene homolog, isoform CRA_a
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Predicted intracellular proteins
RAS pathway related proteins
Cancer-related genes
   Mutated cancer genes
   Mutational cancer driver genes
   COSMIC somatic mutations in cancer genes
   COSMIC Somatic Mutations
   COSMIC Missense Mutations
Disease related genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000139 [Golgi membrane]
GO:0000165 [MAPK cascade]
GO:0000186 [activation of MAPKK activity]
GO:0001932 [regulation of protein phosphorylation]
GO:0002223 [stimulatory C-type lectin receptor signaling pathway]
GO:0005515 [protein binding]
GO:0005525 [GTP binding]
GO:0005622 [intracellular]
GO:0005794 [Golgi apparatus]
GO:0005886 [plasma membrane]
GO:0007165 [signal transduction]
GO:0007173 [epidermal growth factor receptor signaling pathway]
GO:0007264 [small GTPase mediated signal transduction]
GO:0007265 [Ras protein signal transduction]
GO:0007411 [axon guidance]
GO:0007596 [blood coagulation]
GO:0008286 [insulin receptor signaling pathway]
GO:0008543 [fibroblast growth factor receptor signaling pathway]
GO:0010468 [regulation of gene expression]
GO:0015031 [protein transport]
GO:0016020 [membrane]
GO:0032403 [protein complex binding]
GO:0038095 [Fc-epsilon receptor signaling pathway]
GO:0045087 [innate immune response]
GO:0048010 [vascular endothelial growth factor receptor signaling pathway]
GO:0048011 [neurotrophin TRK receptor signaling pathway]
GO:0048146 [positive regulation of fibroblast proliferation]
GO:0048642 [negative regulation of skeletal muscle tissue development]
GO:0050900 [leukocyte migration]
GO:0070062 [extracellular exosome]
GO:0072600 [establishment of protein localization to Golgi]
Show all
189 aa
21.2 kDa
No 0

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