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GSTM2

ANTIBODY INFORMATION
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   NCI-CPTC
  

Product name

 HPA055972   HPA055973   CPTC-GSTMu2-2  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

 GSTM1 - ENSG00000134184 [100%]
GSTM5 - ENSG00000134201 [96%]
GSTM3 - ENSG00000134202 [88%]
GSTM4 - ENSG00000168765 [100%]
   GSTM1 - ENSG00000134184 [100%]
GSTM5 - ENSG00000134201 [81%]
GSTM4 - ENSG00000168765 [100%]
    

Released in version

 13   13   7  

References

      

Proper citation

 Atlas Antibodies Cat#HPA055972, RRID:AB_2682992   Atlas Antibodies Cat#HPA055973, RRID:AB_2682993    

VALIDATION SUMMARY
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  
Immunocytochemistry

    Supported  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  

Standard validation

     Supported  

     

Figure description

     Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.  

Antibody dilution

     1:20  

Literature conformity

     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in myocytes.   Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 16 cell types		   RNA: detected in 37 tissues
Protein: detected in 35 cell types		   RNA: detected in 37 tissues
Protein: detected in 43 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:450   1:15   1:5000  

Literature conformity

 Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

      

Target mass (kDa)

 27.6, 26.6, 26.1, 25.7, 25.6, 24.9, 23.4, 23, 22.9, 22.8, 22.6, 21.4, 21.3, 17.4, 12   27.6, 25.7, 25.6   26.1, 25.7, 22.9, 22.6, 21.4, 17.4, 10.4  

Antibody dilution

 1:310   1:100   1:250  

PROTEIN ARRAY
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:2450   1:1100    

ANTIGEN INFORMATION
 
 

Antibody HPA055972

  

Antibody HPA055973

  

Antibody CAB040547

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 26   27    

Antigen sequence

 
HKITQSNAILCYIARKHNLCGETEEE
  
LKDFISRFEGLEKISAYMKSSRFLPRP
  

											 
																				

																		

										
											
Matching transcripts

												
																					GSTM2-001 - ENSP00000358846 [88%] 
GSTM2-002 - ENSP00000241337 [88%] 
GSTM2-004 - ENSP00000358844 [88%] 
GSTM2-005 - ENSP00000435910 [88%] 
GSTM2-009 - ENSP00000435157 [88%] 
GSTM2-201 - ENSP00000358842 [88%] 
GSTM2-202 - ENSP00000416883 [88%] 

											 
																						GSTM2-002 - ENSP00000241337 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					GSTM1 - ENSG00000134184 [100%]
GSTM5 - ENSG00000134201 [96%]
GSTM3 - ENSG00000134202 [88%]
GSTM4 - ENSG00000168765 [100%]

											 
																						GSTM1 - ENSG00000134184 [100%]
GSTM5 - ENSG00000134201 [81%]
GSTM4 - ENSG00000168765 [100%]

											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
GSTM2-001
GSTM2-002
GSTM2-004
GSTM2-005
GSTM2-009
GSTM2-201
GSTM2-202
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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