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Immunofluorescent staining of human cell line A549 shows localization to vesicles.
Immunofluorescent staining of human cell line A549 shows localization to lipid droplets.
Antibody dilution
1:20
1:22
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA043708
Antibody HPA054586
Antibody HPA069774
Standard validation
Uncertain
Uncertain
Figure description
Immunohistochemical staining of human kidney shows moderate positivity in tubular cells and cells in glomeruli.
Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in endocrine cells.
Expression
RNA: detected in 4 tissues Protein: detected in 45 cell types
RNA: detected in 4 tissues Protein: detected in 23 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:50
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043708
Antibody HPA054586
Antibody HPA069774
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407599)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407599)
Target mass (kDa)
34.2
34.2
34.2
Antibody dilution
1:250
1:125
1:80
PROTEIN ARRAY
Antibody HPA043708
Antibody HPA054586
Antibody HPA069774
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.