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FBXO16

ANTIBODY INFORMATION
 
 

Antibody HPA053955

  

Antibody HPA058676

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053955   HPA058676  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   16  

References

    

Proper citation

 Atlas Antibodies Cat#HPA053955, RRID:AB_2682320   Atlas Antibodies Cat#HPA058676, RRID:AB_2683795  

VALIDATION SUMMARY
 
 

Antibody HPA053955

  

Antibody HPA058676

  
Immunocytochemistry

  Approved  
Immunohistochemistry

Uncertain    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053955

  

Antibody HPA058676

  

Standard validation

   Approved  

   

Figure description

   Immunofluorescent staining of human cell line SK-MEL-30 shows localization to nucleoplasm, plasma membrane, cytosol & vesicles.  

Antibody dilution

   1:29  

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053955

  

Antibody HPA058676

  

Standard validation

 Uncertain    

   

Figure description

 Immunohistochemical staining of human stomach, upper shows strong cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 33 tissues
Protein: detected in 22 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:10    

Literature conformity

 No avaliable gene/protein characterization data.    

RNA consistency

 Not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA053955

  

Antibody HPA058676

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 36.7, 34.6, 33.1   36.7, 34.6, 33.1, 21.3  

Antibody dilution

 1:140   1:100  

PROTEIN ARRAY
 
 

Antibody HPA053955

  

Antibody HPA058676

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1150   1:1050  

ANTIGEN INFORMATION
 
 

Antibody HPA053955

  

Antibody HPA058676

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 79   73  

Antigen sequence

 
DPRSLCRCAQVCWHWKNLAELDQLWMLKCLRFNWYINFSPTPFEQGIWKK
HYIQMVKELHITKPKTPPKDGFVIADVQL
  
TPLNHQLLNDRVFEERRALLGKWFDKWTDSQRRRILTGLLERCSLSQQKF
CCRKLQEKIPAEALDFTTKLPRV
 

Matching transcripts

 FBXO16-001 - ENSP00000369604 [100%]
FBXO16-007 - ENSP00000341416 [100%]
FBXO16-008 - ENSP00000429687 [100%]
  FBXO16-001 - ENSP00000369604 [100%]
FBXO16-005 - ENSP00000429390 [100%]
FBXO16-007 - ENSP00000341416 [84%]
FBXO16-008 - ENSP00000429687 [84%]
 

Other gene match

    
ANTIGEN VIEW
FBXO16-001
FBXO16-005
FBXO16-007
FBXO16-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.