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Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Immunohistochemical staining of human testis shows strong cytoplasmic and nuclear positivity in cells of seminiferus ducts.
Expression
RNA: detected in 1 tissues Protein: detected in 20 cell types
RNA: detected in 1 tissues Protein: detected in 52 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:100
1:100
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA001193
Antibody HPA002820
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406154)
Target mass (kDa)
39
39
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA001193
Antibody HPA002820
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
