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CBWD7

ANTIBODY INFORMATION
 
 

Antibody HPA042759

  

Antibody HPA042813

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042759   HPA042813  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 CBWD2 - ENSG00000136682 [100%]
CBWD5 - ENSG00000147996 [97%]
CBWD1 - ENSG00000172785 [100%]
CBWD3 - ENSG00000196873 [97%]
   CBWD2 - ENSG00000136682 [100%]
CBWD5 - ENSG00000147996 [100%]
CBWD1 - ENSG00000172785 [100%]
CBWD3 - ENSG00000196873 [100%]
  

Released in version

 12   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042759, RRID:AB_2678149   Atlas Antibodies Cat#HPA042813, RRID:AB_2678181  

VALIDATION SUMMARY
 
 

Antibody HPA042759

  

Antibody HPA042813

  
Immunocytochemistry

Approved    
Immunohistochemistry

Approved   Approved  
Western blot

Uncertain   Uncertain  
Protein array

Supported   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042759

  

Antibody HPA042813

  

Standard validation

 Approved    

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.    

Antibody dilution

 1:66    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042759

  

Antibody HPA042813

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human prostate shows strong cytoplasmic and membranous positivity in glandular cells.   Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.  

Expression

 RNA: detected in 13 tissues
Protein: detected in 51 cell types		   RNA: detected in 13 tissues
Protein: detected in 51 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:350   1:125  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042759

  

Antibody HPA042813

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 44.1, 44, 42, 41.9, 40.4, 40.3, 27.5   44.1, 44, 42, 41.9, 40.4, 40.3, 38.8, 38.7, 27.5, 19.6, 19.5, 12.2, 12.1, 8.5  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA042759

  

Antibody HPA042813

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA042759

  

Antibody HPA042813

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 32   34  

Antigen sequence

 
LINEATRQVALADAILINKTDLVPEEDVKKLR
  
EGSALEKSLAVSQGGELYEEWLELRNGCLCCSVK
 

Matching transcripts

 CBWD7-001 - ENSP00000401079 [97%]
CBWD7-005 - ENSP00000366608 [97%]
CBWD7-201 - ENSP00000482804 [97%]
  CBWD7-001 - ENSP00000401079 [100%]
CBWD7-005 - ENSP00000366608 [100%]
CBWD7-201 - ENSP00000482804 [100%]
CBWD7-202 - ENSP00000481300 [100%]
 

Other gene match

 CBWD2 - ENSG00000136682 [100%]
CBWD5 - ENSG00000147996 [97%]
CBWD1 - ENSG00000172785 [100%]
CBWD3 - ENSG00000196873 [97%]
   CBWD2 - ENSG00000136682 [100%]
CBWD5 - ENSG00000147996 [100%]
CBWD1 - ENSG00000172785 [100%]
CBWD3 - ENSG00000196873 [100%]
  
ANTIGEN VIEW
CBWD7-001
CBWD7-005
CBWD7-201
CBWD7-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.