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ANTIBODY INFORMATION
 
 

Antibody HPA036316

  

Antibody HPA036317

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036316   HPA036317  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   7  

References

    

Proper citation

 Atlas Antibodies Cat#HPA036316, RRID:AB_2675056   Atlas Antibodies Cat#HPA036317, RRID:AB_10669787  

VALIDATION SUMMARY
 
 

Antibody HPA036316

  

Antibody HPA036317

  
Immunocytochemistry

Supported    
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036316

  

Antibody HPA036317

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleus & nucleoli.    

Antibody dilution

 1:182    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036316

  

Antibody HPA036317

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human testis shows strong nuclear positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human testis shows strong nuclear positivity in a subset of cells in seminiferus ducts.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 34 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:600   1:100  

Literature conformity

 Partly consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036316

  

Antibody HPA036317

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 61.7, 61.2, 36.2   61.7, 61.2  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA036316

  

Antibody HPA036317

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036316

  

Antibody HPA036317

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 75   55  

Antigen sequence

 
SSSFQVLANGATAASKPLQPLGCVPQSVSPSEQALPPHLEPAWPQGLRHN
SVPGRVGPTEYLSPDMQRQRKTKRK
  
PDIQPLERYWAAHQQLRETDIPQLSQASRLSTQQVLDWFDSRLPQPAEVV
VCLDE
 

Matching transcripts

 HOMEZ-001 - ENSP00000350049 [100%]
HOMEZ-002 - ENSP00000453979 [100%]
HOMEZ-006 - ENSP00000475307 [100%]
  HOMEZ-001 - ENSP00000350049 [100%]
HOMEZ-002 - ENSP00000453979 [100%]
 

Other gene match

    
ANTIGEN VIEW
HOMEZ-001
HOMEZ-002
HOMEZ-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.