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Term
Gene name

Class

Subclass

Chromosome

External id

In atlas

Reliability

Reliability

Reliability

Validation

Method

Validation

Method

Validation

Method

Validation

Location

Searches

Tissue

Cell type

Expression

Tissue

Specificity

Cell line

Specificity

Cancer

Specificity

Tissue

Detectable

Cell line

Detectable

Cancer

Detectable

Cancer

Prognosis

Category

Score

Score

Score

Antibodies

Column

 
 
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DDX3X
  • premium

    TISSUE

  • CELL

  • PATHOLOGY

PATHOLOGY ATLAS

GENE/PROTEIN

Antibody validation

Dictionary

Level of antibody staining/expression





High
Medium
Low
Not detected

GENERAL INFORMATION

Gene name

DDX3X

Gene description

DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked

Protein class

Cancer-related genes
Enzymes
Plasma proteins
Predicted intracellular proteins
Transporters

Predicted localization

Intracellular

Number of transcripts

8
SHOW MORE

HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General nuclear expression.

ANTIBODY IHC RELIABILITY

Data reliability
description

Antibody staining mainly consistent with RNA expression data.

Reliability score

Supported based on 3 antibodies.
HPA001648 , HPA005631 , HPA059585
SHOW MORE

PROGNOSTIC SUMMARY

Gene product is not prognostic.
Show all

RNA EXPRESSION OVERVIEW

TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION

Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Liver cancer

STAINING SUMMARY

HPA001648
HPA005631
HPA059585

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Most cancer cells were weakly stained or negative. Moderate to strong immunoreactivity was observed in testicular and urothelial cancers.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Lymphomas, most gliomas and testicular cancers along with several breast and colorectal cancers displayed moderate cytoplasmic positivity. Remaining cancers were in general weakly stained or negative.

Cancer

Cancer staining

Protein
expression of
normal tissue

Breast cancer
 
Carcinoid
 
Cervical cancer
 
 
Colorectal cancer
 
 
Endometrial cancer
 
 
Glioma
 
Head and neck cancer
 
 
Liver cancer
 
 
Lung cancer
 
 
Lymphoma
 
 
 

Cancer

Cancer staining

Protein
expression of
normal tissue

Melanoma
 
Ovarian cancer
 
Pancreatic cancer
 
Prostate cancer
 
Renal cancer
 
Skin cancer
 
Stomach cancer
 
 
Testis cancer
 
Thyroid cancer
 
Urothelial cancer
 

Staining summary

Moderate to strong nuclear staining in all cancer tissues.

GENE INFORMATION

Gene name

DDX3X (HGNC Symbol)

Synonyms

DBX, DDX14, DDX3, HLP2

Description

DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked (HGNC Symbol)

Entrez gene summary

The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Chromosome

X

Cytoband

p11.4

Chromosome location (bp)

41333348 - 41364472

Protein evidence

Evidence at protein level (all genes)

Ensembl

ENSG00000215301 (version 83.38)

Entrez gene

1654

UniProt

O00571 (UniProt - Evidence at protein level)

neXtProt

NX_O00571

Antibodypedia

DDX3X antibodies

PROTEIN BROWSER

DDX3X-001
DDX3X-005
DDX3X-012
DDX3X-014
DDX3X-017
DDX3X-020
DDX3X-021
DDX3X-201

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)

DDX3X-001
ENSP00000382840
ENST00000399959
O00571 [Direct mapping]
ATP-dependent RNA helicase DDX3X
Show all
Enzymes
   ENZYME proteins
   Hydrolases
Transporters
   Transporter channels and pores
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003676 [nucleic acid binding]
GO:0003677 [DNA binding]
GO:0003723 [RNA binding]
GO:0003924 [GTPase activity]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0004004 [ATP-dependent RNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005741 [mitochondrial outer membrane]
GO:0005852 [eukaryotic translation initiation factor 3 complex]
GO:0006351 [transcription, DNA-templated]
GO:0006413 [translational initiation]
GO:0007059 [chromosome segregation]
GO:0008134 [transcription factor binding]
GO:0008143 [poly(A) binding]
GO:0008190 [eukaryotic initiation factor 4E binding]
GO:0008625 [extrinsic apoptotic signaling pathway via death domain receptors]
GO:0009615 [response to virus]
GO:0010494 [cytoplasmic stress granule]
GO:0010501 [RNA secondary structure unwinding]
GO:0010628 [positive regulation of gene expression]
GO:0016032 [viral process]
GO:0016607 [nuclear speck]
GO:0016887 [ATPase activity]
GO:0017111 [nucleoside-triphosphatase activity]
GO:0017148 [negative regulation of translation]
GO:0022627 [cytosolic small ribosomal subunit]
GO:0030307 [positive regulation of cell growth]
GO:0030308 [negative regulation of cell growth]
GO:0031333 [negative regulation of protein complex assembly]
GO:0031369 [translation initiation factor binding]
GO:0032508 [DNA duplex unwinding]
GO:0032728 [positive regulation of interferon-beta production]
GO:0034063 [stress granule assembly]
GO:0035556 [intracellular signal transduction]
GO:0035613 [RNA stem-loop binding]
GO:0042256 [mature ribosome assembly]
GO:0043024 [ribosomal small subunit binding]
GO:0043065 [positive regulation of apoptotic process]
GO:0043066 [negative regulation of apoptotic process]
GO:0043154 [negative regulation of cysteine-type endopeptidase activity involved in apoptotic process]
GO:0043273 [CTPase activity]
GO:0043280 [positive regulation of cysteine-type endopeptidase activity involved in apoptotic process]
GO:0044822 [poly(A) RNA binding]
GO:0045070 [positive regulation of viral genome replication]
GO:0045087 [innate immune response]
GO:0045727 [positive regulation of translation]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0045948 [positive regulation of translational initiation]
GO:0048027 [mRNA 5'-UTR binding]
GO:0070062 [extracellular exosome]
GO:0071243 [cellular response to arsenic-containing substance]
GO:0071470 [cellular response to osmotic stress]
GO:0071651 [positive regulation of chemokine (C-C motif) ligand 5 production]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1900087 [positive regulation of G1/S transition of mitotic cell cycle]
GO:2001243 [negative regulation of intrinsic apoptotic signaling pathway]
Show all
662 aa
73.2 kDa
No 0
DDX3X-005
ENSP00000414281
ENST00000441189
B4DLA0 [Direct mapping]
ATP-dependent RNA helicase DDX3X; cDNA FLJ55031, highly similar to ATP-dependent RNA helicase DDX3X (EC 3.6.1.-)
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004386 [helicase activity]
GO:0006413 [translational initiation]
Show all
139 aa
15 kDa
No 0
DDX3X-012
ENSP00000479790
ENST00000611968
A0A087WVZ1 [Direct mapping]
ATP-dependent RNA helicase DDX3X
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0006413 [translational initiation]
Show all
138 aa
14.7 kDa
No 0
DDX3X-014
ENSP00000487224
ENST00000629496
O00571 [Direct mapping]
ATP-dependent RNA helicase DDX3X
Show all
Enzymes
   ENZYME proteins
   Hydrolases
Transporters
   Transporter channels and pores
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003676 [nucleic acid binding]
GO:0003677 [DNA binding]
GO:0003723 [RNA binding]
GO:0003924 [GTPase activity]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0004004 [ATP-dependent RNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005741 [mitochondrial outer membrane]
GO:0005852 [eukaryotic translation initiation factor 3 complex]
GO:0006351 [transcription, DNA-templated]
GO:0006413 [translational initiation]
GO:0007059 [chromosome segregation]
GO:0008134 [transcription factor binding]
GO:0008143 [poly(A) binding]
GO:0008190 [eukaryotic initiation factor 4E binding]
GO:0008625 [extrinsic apoptotic signaling pathway via death domain receptors]
GO:0009615 [response to virus]
GO:0010494 [cytoplasmic stress granule]
GO:0010501 [RNA secondary structure unwinding]
GO:0010628 [positive regulation of gene expression]
GO:0016032 [viral process]
GO:0016607 [nuclear speck]
GO:0016887 [ATPase activity]
GO:0017111 [nucleoside-triphosphatase activity]
GO:0017148 [negative regulation of translation]
GO:0022627 [cytosolic small ribosomal subunit]
GO:0030307 [positive regulation of cell growth]
GO:0030308 [negative regulation of cell growth]
GO:0031333 [negative regulation of protein complex assembly]
GO:0031369 [translation initiation factor binding]
GO:0032508 [DNA duplex unwinding]
GO:0032728 [positive regulation of interferon-beta production]
GO:0034063 [stress granule assembly]
GO:0035556 [intracellular signal transduction]
GO:0035613 [RNA stem-loop binding]
GO:0042256 [mature ribosome assembly]
GO:0043024 [ribosomal small subunit binding]
GO:0043065 [positive regulation of apoptotic process]
GO:0043066 [negative regulation of apoptotic process]
GO:0043154 [negative regulation of cysteine-type endopeptidase activity involved in apoptotic process]
GO:0043273 [CTPase activity]
GO:0043280 [positive regulation of cysteine-type endopeptidase activity involved in apoptotic process]
GO:0044822 [poly(A) RNA binding]
GO:0045070 [positive regulation of viral genome replication]
GO:0045087 [innate immune response]
GO:0045727 [positive regulation of translation]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0045948 [positive regulation of translational initiation]
GO:0048027 [mRNA 5'-UTR binding]
GO:0070062 [extracellular exosome]
GO:0071243 [cellular response to arsenic-containing substance]
GO:0071470 [cellular response to osmotic stress]
GO:0071651 [positive regulation of chemokine (C-C motif) ligand 5 production]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1900087 [positive regulation of G1/S transition of mitotic cell cycle]
GO:2001243 [negative regulation of intrinsic apoptotic signaling pathway]
Show all
662 aa
73.2 kDa
No 0
DDX3X-017
ENSP00000486306
ENST00000625837
A0A0D9SF53 [Direct mapping]
ATP-dependent RNA helicase DDX3X
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003676 [nucleic acid binding]
GO:0004386 [helicase activity]
GO:0005524 [ATP binding]
GO:0006413 [translational initiation]
Show all
733 aa
81.5 kDa
No 0
DDX3X-020
ENSP00000486443
ENST00000626301
A0A0D9SFB3 [Direct mapping]
ATP-dependent RNA helicase DDX3X
Show all
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003676 [nucleic acid binding]
GO:0004386 [helicase activity]
GO:0005524 [ATP binding]
GO:0006413 [translational initiation]
Show all
640 aa
70.8 kDa
No 0
DDX3X-021
ENSP00000488854
ENST00000631641
Predicted intracellular proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Ezkurdia et al 2014)
Show all
164 aa
17.8 kDa
No 0
DDX3X-201
ENSP00000392494
ENST00000457138
O00571 [Direct mapping]
ATP-dependent RNA helicase DDX3X
Show all
Enzymes
   ENZYME proteins
   Hydrolases
Transporters
   Transporter channels and pores
Predicted intracellular proteins
Plasma proteins
Cancer-related genes
   Mutated cancer genes
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0003676 [nucleic acid binding]
GO:0003677 [DNA binding]
GO:0003723 [RNA binding]
GO:0003924 [GTPase activity]
GO:0004003 [ATP-dependent DNA helicase activity]
GO:0004004 [ATP-dependent RNA helicase activity]
GO:0005515 [protein binding]
GO:0005524 [ATP binding]
GO:0005634 [nucleus]
GO:0005737 [cytoplasm]
GO:0005741 [mitochondrial outer membrane]
GO:0005852 [eukaryotic translation initiation factor 3 complex]
GO:0006351 [transcription, DNA-templated]
GO:0006413 [translational initiation]
GO:0007059 [chromosome segregation]
GO:0008134 [transcription factor binding]
GO:0008143 [poly(A) binding]
GO:0008190 [eukaryotic initiation factor 4E binding]
GO:0008625 [extrinsic apoptotic signaling pathway via death domain receptors]
GO:0009615 [response to virus]
GO:0010494 [cytoplasmic stress granule]
GO:0010501 [RNA secondary structure unwinding]
GO:0010628 [positive regulation of gene expression]
GO:0016032 [viral process]
GO:0016607 [nuclear speck]
GO:0016887 [ATPase activity]
GO:0017111 [nucleoside-triphosphatase activity]
GO:0017148 [negative regulation of translation]
GO:0022627 [cytosolic small ribosomal subunit]
GO:0030307 [positive regulation of cell growth]
GO:0030308 [negative regulation of cell growth]
GO:0031333 [negative regulation of protein complex assembly]
GO:0031369 [translation initiation factor binding]
GO:0032508 [DNA duplex unwinding]
GO:0032728 [positive regulation of interferon-beta production]
GO:0034063 [stress granule assembly]
GO:0035556 [intracellular signal transduction]
GO:0035613 [RNA stem-loop binding]
GO:0042256 [mature ribosome assembly]
GO:0043024 [ribosomal small subunit binding]
GO:0043065 [positive regulation of apoptotic process]
GO:0043066 [negative regulation of apoptotic process]
GO:0043154 [negative regulation of cysteine-type endopeptidase activity involved in apoptotic process]
GO:0043273 [CTPase activity]
GO:0043280 [positive regulation of cysteine-type endopeptidase activity involved in apoptotic process]
GO:0044822 [poly(A) RNA binding]
GO:0045070 [positive regulation of viral genome replication]
GO:0045087 [innate immune response]
GO:0045727 [positive regulation of translation]
GO:0045944 [positive regulation of transcription from RNA polymerase II promoter]
GO:0045948 [positive regulation of translational initiation]
GO:0048027 [mRNA 5'-UTR binding]
GO:0070062 [extracellular exosome]
GO:0071243 [cellular response to arsenic-containing substance]
GO:0071470 [cellular response to osmotic stress]
GO:0071651 [positive regulation of chemokine (C-C motif) ligand 5 production]
GO:0097193 [intrinsic apoptotic signaling pathway]
GO:1900087 [positive regulation of G1/S transition of mitotic cell cycle]
GO:2001243 [negative regulation of intrinsic apoptotic signaling pathway]
Show all
646 aa
71.4 kDa
No 0

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