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DHFR

GENERAL INFORMATION

Gene name

 DHFR

Gene description

 Dihydrofolate reductase

Protein class

 Cancer-related genes
Disease related genes
Enzymes
FDA approved drug targets
Predicted intracellular proteins

Predicted localization

 Intracellular

Number of transcripts

 4
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HUMAN PROTEIN ATLAS INFORMATION

RNA category
TCGA (cancer tissue):Expressed in all
HPA (cell line):Expressed in all
HPA (normal tissue):Mixed

Protein evidence

 Evidence at protein level

Protein expression
normal tissue

 General cytoplasmic expression with a granular pattern.

ANTIBODY IHC RELIABILITY

Data reliability
description

 Antibody staining mainly not consistent with RNA expression data. Caution, targets protein from more than one gene.

Reliability score

 Uncertain based on 2 antibodies.
HPA051465 , CAB037129
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PROGNOSTIC SUMMARY
Gene product is not prognostic.
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RNA EXPRESSION OVERVIEW
TCGA dataset
RNA cancer category: Expressed in all

PROTEIN EXPRESSION
Colorectal cancer
Breast cancer
Prostate cancer
Lung cancer
Skin cancer

STAINING SUMMARY
HPA051465
CAB037129

GENE INFORMATION

Gene name

 DHFR

Synonyms

Description

 Dihydrofolate reductase (HGNC Symbol)

Entrez gene summary

 Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

Chromosome

 5

Cytoband

 q14.1

Chromosome location (bp)

 80626228 - 80654983

Protein evidence

 Evidence at protein level (all genes)

Ensembl

 ENSG00000228716 (version 83.38)

Entrez gene

 1719

UniProt

 P00374 (UniProt - Evidence at protein level)

neXtProt

 NX_P00374

Antibodypedia

 DHFR antibodies

PROTEIN BROWSER
DHFR-001
DHFR-003
DHFR-005
DHFR-006

PROTEIN INFORMATION

Splice variant

UniProt

Protein class

Gene ontology

Length & mass

Signal peptide
(predicted)

Transmembrane regions
(predicted)
DHFR-001
ENSP00000396308
ENST00000439211
P00374 [Direct mapping]
Dihydrofolate reductase
B0YJ76 [Target identity:100%; Query identity:100%]
Dihydrofolate reductase; cDNA, FLJ93028, Homo sapiens dihydrofolate reductase (DHFR), mRNA
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Enzymes
   ENZYME proteins
   Oxidoreductases
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Kim et al 2014)
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000083 [regulation of transcription involved in G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0003729 [mRNA binding]
GO:0004146 [dihydrofolate reductase activity]
GO:0005542 [folic acid binding]
GO:0005575 [cellular_component]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006545 [glycine biosynthetic process]
GO:0006729 [tetrahydrobiopterin biosynthetic process]
GO:0006730 [one-carbon metabolic process]
GO:0006766 [vitamin metabolic process]
GO:0006767 [water-soluble vitamin metabolic process]
GO:0008144 [drug binding]
GO:0009165 [nucleotide biosynthetic process]
GO:0031103 [axon regeneration]
GO:0031427 [response to methotrexate]
GO:0044281 [small molecule metabolic process]
GO:0046209 [nitric oxide metabolic process]
GO:0046452 [dihydrofolate metabolic process]
GO:0046653 [tetrahydrofolate metabolic process]
GO:0046654 [tetrahydrofolate biosynthetic process]
GO:0046655 [folic acid metabolic process]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0051000 [positive regulation of nitric-oxide synthase activity]
GO:0051870 [methotrexate binding]
GO:0055114 [oxidation-reduction process]
GO:0070402 [NADPH binding]
GO:2000121 [regulation of removal of superoxide radicals]
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 187 aa
21.5 kDa 		No 0
DHFR-003
ENSP00000421334
ENST00000504396
P00374 [Direct mapping]
Dihydrofolate reductase
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Enzymes
   ENZYME proteins
   Oxidoreductases
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000083 [regulation of transcription involved in G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0003729 [mRNA binding]
GO:0004146 [dihydrofolate reductase activity]
GO:0005542 [folic acid binding]
GO:0005575 [cellular_component]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006545 [glycine biosynthetic process]
GO:0006729 [tetrahydrobiopterin biosynthetic process]
GO:0006730 [one-carbon metabolic process]
GO:0006766 [vitamin metabolic process]
GO:0006767 [water-soluble vitamin metabolic process]
GO:0008144 [drug binding]
GO:0009165 [nucleotide biosynthetic process]
GO:0031103 [axon regeneration]
GO:0031427 [response to methotrexate]
GO:0044281 [small molecule metabolic process]
GO:0046209 [nitric oxide metabolic process]
GO:0046452 [dihydrofolate metabolic process]
GO:0046653 [tetrahydrofolate metabolic process]
GO:0046654 [tetrahydrofolate biosynthetic process]
GO:0046655 [folic acid metabolic process]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0051000 [positive regulation of nitric-oxide synthase activity]
GO:0051870 [methotrexate binding]
GO:0055114 [oxidation-reduction process]
GO:0070402 [NADPH binding]
GO:2000121 [regulation of removal of superoxide radicals]
Show all
 135 aa
15.7 kDa 		No 0
DHFR-005
ENSP00000422732
ENST00000511032
B4DM58 [Direct mapping]
Dihydrofolate reductase; cDNA FLJ51500, highly similar to Dihydrofolate reductase (EC 1.5.1.3)
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   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0004146 [dihydrofolate reductase activity]
GO:0006545 [glycine biosynthetic process]
GO:0009165 [nucleotide biosynthetic process]
GO:0050661 [NADP binding]
GO:0055114 [oxidation-reduction process]
Show all
 129 aa
14.5 kDa 		No 0
DHFR-006
ENSP00000426474
ENST00000505337
P00374 [Direct mapping]
Dihydrofolate reductase
B0YJ76 [Target identity:100%; Query identity:100%]
Dihydrofolate reductase; cDNA, FLJ93028, Homo sapiens dihydrofolate reductase (DHFR), mRNA
Show all
Enzymes
   ENZYME proteins
   Oxidoreductases
   SPOCTOPUS predicted secreted proteins
Predicted intracellular proteins
Cancer-related genes
   Candidate cancer biomarkers
Disease related genes
FDA approved drug targets
   Small molecule drugs
Protein evidence (Ezkurdia et al 2014)
Show all
GO:0000082 [G1/S transition of mitotic cell cycle]
GO:0000083 [regulation of transcription involved in G1/S transition of mitotic cell cycle]
GO:0000278 [mitotic cell cycle]
GO:0003729 [mRNA binding]
GO:0004146 [dihydrofolate reductase activity]
GO:0005542 [folic acid binding]
GO:0005575 [cellular_component]
GO:0005654 [nucleoplasm]
GO:0005829 [cytosol]
GO:0006545 [glycine biosynthetic process]
GO:0006729 [tetrahydrobiopterin biosynthetic process]
GO:0006730 [one-carbon metabolic process]
GO:0006766 [vitamin metabolic process]
GO:0006767 [water-soluble vitamin metabolic process]
GO:0008144 [drug binding]
GO:0009165 [nucleotide biosynthetic process]
GO:0031103 [axon regeneration]
GO:0031427 [response to methotrexate]
GO:0044281 [small molecule metabolic process]
GO:0046209 [nitric oxide metabolic process]
GO:0046452 [dihydrofolate metabolic process]
GO:0046653 [tetrahydrofolate metabolic process]
GO:0046654 [tetrahydrofolate biosynthetic process]
GO:0046655 [folic acid metabolic process]
GO:0050999 [regulation of nitric-oxide synthase activity]
GO:0051000 [positive regulation of nitric-oxide synthase activity]
GO:0051870 [methotrexate binding]
GO:0055114 [oxidation-reduction process]
GO:0070402 [NADPH binding]
GO:2000121 [regulation of removal of superoxide radicals]
Show all
 187 aa
21.5 kDa 		No 0

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.