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Immunofluorescent staining of human cell line U-251 MG shows localization to centrosome.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA055997
Antibody HPA062674
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human lymph node shows strong nuclear and cytoplasmic positivity in a subset of germinal center cells.
Immunohistochemical staining of human tonsil shows nuclear positivity.
Expression
RNA: detected in 7 tissues Protein: detected in 46 cell types
RNA: detected in 7 tissues Protein: detected in 35 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1200
1:900
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA055997
Antibody HPA062674
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
73.7
73.7
Antibody dilution
1:1750
1:370
PROTEIN ARRAY
Antibody HPA055997
Antibody HPA062674
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
