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SERPINB10

ANTIBODY INFORMATION
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA015480   HPA043595   HPA059582  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 SERPINB2 - ENSG00000197632 [100%]
      

Released in version

 4   16   13  

References

      

Proper citation

 Atlas Antibodies Cat#HPA015480, RRID:AB_1855456   Atlas Antibodies Cat#HPA043595, RRID:AB_2678572   Atlas Antibodies Cat#HPA059582, RRID:AB_2684072  

VALIDATION SUMMARY
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  
Immunocytochemistry

Approved   Supported    
Immunohistochemistry

Uncertain     Uncertain  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Approved   Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  

Standard validation

 Approved   Supported    

     

Figure description

 Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.   Immunofluorescent staining of human cell line HaCaT shows localization to nucleus & cytosol.    

Antibody dilution

 1:60   1:9    

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  

Standard validation

 Uncertain     Uncertain  

     

Figure description

 Immunohistochemical staining of human esophagus shows cytoplasmic positivity in squamous epithelial cells.     Immunohistochemical staining of human appendix shows positivity in a subset of inflammatory cells.  

Expression

 RNA: detected in 2 tissues
Protein: detected in 23 cell types		     RNA: detected in 2 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6     HIER pH6  

Antibody dilution

 1:600     1:300  

Literature conformity

 No avaliable gene/protein characterization data.     No avaliable gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.     Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 46.6, 31.4, 28.5   45.4   45.4  

Antibody dilution

 1:250   1:250   1:280  

PROTEIN ARRAY
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:500   1:3400  

ANTIGEN INFORMATION
 
 

Antibody HPA015480

  

Antibody HPA043595

  

Antibody HPA059582

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 124   76   71  

Antigen sequence

 
TQTKGKIPNLLPEGSVDGDTRMVLVNAVYFKGKWKTPFEKKLNGLYPFRV
NSAQRTPVQMMYLREKLNIGYIEDLKAQILELPYAGDVSMFLLLPDEIAD
VSTGLELLESEITYDKLNKWTSKD
  
LQLYYKSCDLSLLILLPEDINGLEQLEKAITYEKLNEWTSADMMELYEVQ
LHLPKFKLEDSYDLKSTLSSMGMSDA
  
INSWVERQTEGKIQNLLPDDSVDSTTRMILVNALYFKGIWEHQFLVQNTT
EKPFRINETTSKPVQMMFMKK
 

Matching transcripts

 SERPINB10-002 - ENSP00000381082 [100%]
SERPINB10-003 - ENSP00000392381 [100%]
  SERPINB10-001 - ENSP00000238508 [99%]
SERPINB10-201 - ENSP00000479385 [99%]
  SERPINB10-001 - ENSP00000238508 [100%]
SERPINB10-201 - ENSP00000479385 [100%]
 

Other gene match

 SERPINB2 - ENSG00000197632 [100%]
      
ANTIGEN VIEW
SERPINB10-001
SERPINB10-002
SERPINB10-003
SERPINB10-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.