Expression of DDOST in cancer - Summary

GENERAL INFORMATION
Gene name	DDOST
Gene description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic)
Protein class	Disease related genes Enzymes Plasma proteins Potential drug targets Predicted membrane proteins Predicted secreted proteins
Predicted localization	Membrane,Secreted
Number of transcripts	4
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HUMAN PROTEIN ATLAS INFORMATION

RNA category

TCGA (cancer tissue):	Expressed in all
HPA (cell line):	Expressed in all
HPA (normal tissue):	Expressed in all

Protein evidence

Evidence at protein level

Protein expression
normal tissue

General cytoplasmic expression, high expression in glandular cells together with immune cells.

ANTIBODY IHC RELIABILITY
Data reliability description	Antibody staining mainly consistent with RNA expression data. Paired antibodies with high similarity support the protein profile.
Reliability score	Supported based on 3 antibodies. HPA046841 , HPA052867 , CAB009746
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PROGNOSTIC SUMMARY
Prognostic marker in renal cancer (unfavourable), liver cancer (unfavourable), head and neck cancer (unfavourable) and endometrial cancer (favourable).
Renal cancer p<0.001 Liver cancer p<0.001 Head and neck cancer p<0.001 Endometrial cancer p<0.001 Show all

RNA EXPRESSION OVERVIEW
TCGA dataset RNA cancer category: Expressed in all

PROTEIN EXPRESSION

STAINING SUMMARY

HPA046841	HPA052867	CAB009746

GENE INFORMATION
Gene name	DDOST (HGNC Symbol)
Synonyms	KIAA0115, OST, OST48, WBP1
Description	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit (non-catalytic) (HGNC Symbol)
Entrez gene summary	This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia. [provided by RefSeq, Jul 2008]
Chromosome	1
Cytoband	p36.12
Chromosome location (bp)	20651767 - 20661544
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000244038 (version 83.38)
Entrez gene	1650
UniProt	P39656 (UniProt - Evidence at protein level)
neXtProt	NX_P39656
Antibodypedia	DDOST antibodies

PROTEIN BROWSER

DDOST-001	DDOST-005	DDOST-006	DDOST-201

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
DDOST-001 ENSP00000364188 ENST00000375048	P39656 [Direct mapping] Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Show all	Enzymes ENZYME proteins Transferases Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Disease related genes Potential drug targets Protein evidence (Ezkurdia et al 2014) Show all	GO:0004576 [oligosaccharyl transferase activity] GO:0004579 [dolichyl-diphosphooligosaccharide-protein glycotransferase activity] GO:0005515 [protein binding] GO:0005783 [endoplasmic reticulum] GO:0005789 [endoplasmic reticulum membrane] GO:0006412 [translation] GO:0006486 [protein glycosylation] GO:0006487 [protein N-linked glycosylation] GO:0006614 [SRP-dependent cotranslational protein targeting to membrane] GO:0008250 [oligosaccharyltransferase complex] GO:0010467 [gene expression] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0018279 [protein N-linked glycosylation via asparagine] GO:0034097 [response to cytokine] GO:0042110 [T cell activation] GO:0043231 [intracellular membrane-bounded organelle] GO:0043234 [protein complex] GO:0043687 [post-translational protein modification] GO:0044267 [cellular protein metabolic process] GO:0045087 [innate immune response] Show all	456 aa 50.8 kDa	Yes	1
DDOST-005 ENSP00000475634 ENST00000464364	U3KQ84 [Direct mapping] Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Show all	MEMSAT3 predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins Predicted secreted proteins Secreted proteins predicted by MDSEC SignalP predicted secreted proteins Phobius predicted secreted proteins SPOCTOPUS predicted secreted proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004579 [dolichyl-diphosphooligosaccharide-protein glycotransferase activity] GO:0005789 [endoplasmic reticulum membrane] GO:0016021 [integral component of membrane] GO:0018279 [protein N-linked glycosylation via asparagine] Show all	155 aa 17.2 kDa	Yes	0
DDOST-006 ENSP00000473655 ENST00000602624	A0A0C4DGS1 [Direct mapping] Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Ezkurdia et al 2014) Show all	GO:0004579 [dolichyl-diphosphooligosaccharide-protein glycotransferase activity] GO:0005789 [endoplasmic reticulum membrane] GO:0016021 [integral component of membrane] GO:0018279 [protein N-linked glycosylation via asparagine] Show all	439 aa 48.8 kDa	Yes	1
DDOST-201 ENSP00000399457 ENST00000415136	P39656 [Direct mapping] Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit Show all	Enzymes ENZYME proteins Transferases Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Plasma proteins Disease related genes Potential drug targets Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0004576 [oligosaccharyl transferase activity] GO:0004579 [dolichyl-diphosphooligosaccharide-protein glycotransferase activity] GO:0005515 [protein binding] GO:0005783 [endoplasmic reticulum] GO:0005789 [endoplasmic reticulum membrane] GO:0006412 [translation] GO:0006486 [protein glycosylation] GO:0006487 [protein N-linked glycosylation] GO:0006614 [SRP-dependent cotranslational protein targeting to membrane] GO:0008250 [oligosaccharyltransferase complex] GO:0010467 [gene expression] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0018279 [protein N-linked glycosylation via asparagine] GO:0034097 [response to cytokine] GO:0042110 [T cell activation] GO:0043231 [intracellular membrane-bounded organelle] GO:0043234 [protein complex] GO:0043687 [post-translational protein modification] GO:0044267 [cellular protein metabolic process] GO:0045087 [innate immune response] Show all	456 aa 50.8 kDa	Yes	1