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Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Antibody dilution
1:89
1:143
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA052734
Antibody HPA065037
Antibody HPA067937
Standard validation
Approved
Figure description
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 30 cell types
Retrieval
HIER pH6
Antibody dilution
1:2500
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA052734
Antibody HPA065037
Antibody HPA067937
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
37.6
37.6
37.6
Antibody dilution
1:450
1:300
1:480
PROTEIN ARRAY
Antibody HPA052734
Antibody HPA065037
Antibody HPA067937
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
