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YJEFN3

ANTIBODY INFORMATION
 
 

Antibody HPA060789

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA060789  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

Proper citation

 Atlas Antibodies Cat#HPA060789, RRID:AB_2684366  

VALIDATION SUMMARY
 
 

Antibody HPA060789

  
Immunocytochemistry

Approved  
Immunohistochemistry

Approved  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA060789

  

Standard validation

 Approved  

 

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.  

Antibody dilution

 1:32  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA060789

  

Standard validation

 Approved  

 

Figure description

 Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in a rare subset of neurons.  

Expression

 RNA: detected in 4 tissues
Protein: detected in 8 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:80  

Literature conformity

 Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA060789

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

 

Figure description

  

Target mass (kDa)

 32.6, 27  

Antibody dilution

 1:110  

PROTEIN ARRAY
 
 

Antibody HPA060789

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1150  

ANTIGEN INFORMATION
 
 

Antibody HPA060789

  

Antigen

 Recombinant protein fragment  

Length (aa)

 64  

Antigen sequence

 
SGWDAETGSDSEDGLRPDVLVSLAAPKRCAGRFSGRHHFVAGRFVPDDVR
RKFALRLPGYTGTD
 

Matching transcripts

 YJEFN3-001 - ENSP00000426964 [100%]
YJEFN3-003 - ENSP00000398520 [100%]
 

Other gene match

  
ANTIGEN VIEW
YJEFN3-001
YJEFN3-003
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.