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Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & vesicles.
Antibody dilution
1:93
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA047220
Antibody HPA047851
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in islets of Langerhans.
Expression
RNA: detected in 0 tissues Protein: detected in 46 cell types
Retrieval
HIER pH6
Antibody dilution
1:900
Literature conformity
RNA consistency
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA047220
Antibody HPA047851
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
165.1, 82.9, 64.8
165.1, 82.9, 64.8, 20.3
Antibody dilution
1:470
1:320
PROTEIN ARRAY
Antibody HPA047220
Antibody HPA047851
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
