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Immunohistochemical staining of human colon shows moderate cytoplasmic and nuclear positivity in glandular cells.
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human esophagus shows strong cytoplasmic and membranous positivity in squamous epithelial cells.
Immunohistochemical staining of human kidney shows strong cytoplasmic and membranous positivity in cells in tubules. Moderate positivity was observed in cells in glomeruli.
Immunohistochemical staining of human stomach, upper shows strong membranous positivity in glandular cells.
Expression
RNA: detected in 0 tissues Protein: detected in 63 cell types
RNA: detected in 0 tissues Protein: detected in 54 cell types
RNA: detected in 0 tissues Protein: detected in 33 cell types
RNA: detected in 0 tissues Protein: detected in 54 cell types
RNA: detected in 0 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:400
1:15
1:50
1:75
1:75
Literature conformity
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
No avaliable gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043239
Antibody HPA047112
Antibody HPA047167
Antibody HPA047766
Antibody HPA051753
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Target mass (kDa)
94.1, 94
94.1, 94
94.1, 94
94.1, 94
94.1, 94
Antibody dilution
1:250
1:80
1:100
1:310
1:230
PROTEIN ARRAY
Antibody HPA043239
Antibody HPA047112
Antibody HPA047167
Antibody HPA047766
Antibody HPA051753
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:6000
1:500
1:2350
1:5000
1:1850
ANTIGEN INFORMATION
Antibody HPA043239
Antibody HPA047112
Antibody HPA047167
Antibody HPA047766
Antibody HPA051753
Antigen
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
51
25
25
31
33
Antigen sequence
KGNRIVNGQGEGPPAKHPSLKPSTEVEDPAVKGAVQRKNVQTLRAEQALP
V