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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in subsets of neuronal cells.
Expression
RNA: detected in 15 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Standard validation
Supported
Figure description
Cytoplasmic staining in neurons as well as axonal and nerve bundle positivity was observed in several regions.
WESTERN BLOT
Antibody HPA014850
Antibody HPA015021
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416829)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
61.5, 32.6
61.5, 32.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA014850
Antibody HPA015021
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
