Fields »
ABOUT
HELP
BLOG
PSMB3

ANTIBODY INFORMATION
 
 

Antibody HPA042775

  

Antibody HPA048147

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042775   HPA048147  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   12  

References

    

Proper citation

 Atlas Antibodies Cat#HPA042775, RRID:AB_2678158   Atlas Antibodies Cat#HPA048147, RRID:AB_2680285  

VALIDATION SUMMARY
 
 

Antibody HPA042775

  

Antibody HPA048147

  
Immunohistochemistry


Validated
  

Validated
  
Western blot

Supported   Uncertain  
Protein array

Supported   Approved  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042775

  

Antibody HPA048147

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA048147 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA042775 across 46 cell lines.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human breast shows strong cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human stomach, lower shows strong cytoplasmic positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 67 cell types		   RNA: detected in 37 tissues
Protein: detected in 61 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:900   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042775

  

Antibody HPA048147

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 22.9   22.9, 16.2  

Antibody dilution

 1:250   1:240  

PROTEIN ARRAY
 
 

Antibody HPA042775

  

Antibody HPA048147

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:1900  

ANTIGEN INFORMATION
 
 

Antibody HPA042775

  

Antibody HPA048147

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 84   80  

Antigen sequence

 
LYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAE
QMYGMCESLWEPNMDPDHLFETISQAMLNAVDRD
  
AMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQT
VAQRLKFRLNLYELKEGRQIKPYTLMSMVA
 

Matching transcripts

 PSMB3-001 - ENSP00000483688 [100%]
  PSMB3-001 - ENSP00000483688 [100%]
PSMB3-006 - ENSP00000478737 [100%]
 

Other gene match

    
ANTIGEN VIEW
PSMB3-001
PSMB3-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.