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Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA038922
Antibody HPA049176
Antibody HPA053326
Antibody CAB016385
Antibody CAB016769
Standard validation
Supported
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human lung shows distinct positivity in plasma.
Immunohistochemical staining of human small intestine shows strong plasma positivity.
Immunohistochemical staining of human ovary shows distinct positivity in plasma.
Immunohistochemical staining of human duodenum shows distinct positivity in plasma.
Immunohistochemical staining of human duodenum shows distinct positivity in plasma.
Expression
RNA: detected in 36 tissues Protein: detected in 34 cell types
RNA: detected in 36 tissues Protein: detected in 17 cell types
RNA: detected in 36 tissues Protein: detected in 5 cell types
RNA: detected in 36 tissues Protein: detected in 23 cell types
RNA: detected in 36 tissues Protein: detected in 3 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:50
1:450
1:80
1:50
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA038922
Antibody HPA049176
Antibody HPA053326
Antibody CAB016385
Antibody CAB016769
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
139.1, 37.7, 30.9
139.1, 51, 43.8
139.1
139.1, 51, 43.8
139.1, 51, 43.8
Antibody dilution
1:250
1:320
1:160
1:500
1:500
PROTEIN ARRAY
Antibody HPA038922
Antibody HPA049176
Antibody HPA053326
Antibody CAB016385
Antibody CAB016769
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
