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CFHR1

ANTIBODY INFORMATION
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA038915   HPA038922   HPA040726  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 CFHR2 - ENSG00000080910 [100%]
CFHR5 - ENSG00000134389 [93%]
RP4-608O15.3 - ENSG00000276911 [100%]
   CFH - ENSG00000000971 [97%]
   CFHR2 - ENSG00000080910 [98%]
CFHR5 - ENSG00000134389 [98%]
RP4-608O15.3 - ENSG00000276911 [98%]
  

Released in version

 10   10   10  

References

      

Proper citation

 Atlas Antibodies Cat#HPA038915, RRID:AB_2676269   Atlas Antibodies Cat#HPA038922, RRID:AB_2676271   Atlas Antibodies Cat#HPA040726, RRID:AB_2677101  

VALIDATION SUMMARY
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  
Immunocytochemistry

  Approved    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  

Standard validation

   Approved    

     

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.    

Antibody dilution

   1:25    

Literature conformity

   The subcellular location is partly supported by literature or no literature is available.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human testis shows positivity in plasma.   Immunohistochemical staining of human lung shows distinct positivity in plasma.   Immunohistochemical staining of human lung shows distinct positivity in plasma.  

Expression

 RNA: detected in 6 tissues
Protein: detected in 15 cell types		   RNA: detected in 6 tissues
Protein: detected in 34 cell types		   RNA: detected in 6 tissues
Protein: detected in 16 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:10   1:50   1:20  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 64.4, 37.7, 30.9, 30.7, 28.9   139.1, 37.7, 30.9   64.4, 37.7, 30.9, 30.7, 28.9  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA038915

  

Antibody HPA038922

  

Antibody HPA040726

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 29   37   40  

Antigen sequence

 
HLEGDTVQIICNTGYRLQNNENNISCVER
  
TGESAEFVCKRGYRLSSRSHTLRTTCWDGKLEYPTCA
  
YKPFSQVPTGEVFYYSCEYNFVSPSKSFWTRITCTEEGWS
 

Matching transcripts

 CFHR1-001 - ENSP00000314299 [100%]
CFHR1-002 - ENSP00000356394 [100%]
  CFHR1-001 - ENSP00000314299 [100%]
CFHR1-002 - ENSP00000356394 [100%]
  CFHR1-001 - ENSP00000314299 [100%]
CFHR1-002 - ENSP00000356394 [100%]
 

Other gene match

 CFHR2 - ENSG00000080910 [100%]
CFHR5 - ENSG00000134389 [93%]
RP4-608O15.3 - ENSG00000276911 [100%]
   CFH - ENSG00000000971 [97%]
   CFHR2 - ENSG00000080910 [98%]
CFHR5 - ENSG00000134389 [98%]
RP4-608O15.3 - ENSG00000276911 [98%]
  
ANTIGEN VIEW
CFHR1-001
CFHR1-002
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.