Cell atlas - CYP51A1 - The Human Protein Atlas

GENERAL INFORMATION
Gene name	CYP51A1
Gene description	Cytochrome P450, family 51, subfamily A, polypeptide 1
Protein class	Enzymes FDA approved drug targets Predicted intracellular proteins Predicted membrane proteins
Predicted localization	Intracellular,Membrane
Number of transcripts	4
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HUMAN PROTEIN ATLAS INFORMATION
Summary	Localized to the endoplasmic reticulum.
RNA cell category	Expressed in all
Protein evidence	Evidence at protein level
Main location	Localized to the Endoplasmic reticulum (validated)
Mouse	Localized to the Endoplasmic reticulum in mouse cells.

DATA RELIABILITY
Reliability score	Validated based on 2 antibodies. HPA041325 , HPA043508
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ASSAYS

RNA EXPRESSION OVERVIEW
RNA cell line category: Expressed in all Organ Origin Category Expression Alphabetical Cell lines sorted after organ of phenotypic resemblance. Cell lines sorted after biological source for establishment. Cell lines sorted after the cell line category according to Cellosaurus. Cell lines sorted on descending RNA expression. Cell lines sorted alphabetically.

HUMAN CELLS

Summary

Localized to the endoplasmic reticulum.

Main location

Endoplasmic reticulum (validated)


	Toggle channels	Low High G1 S G2 M N/A

Antibody	Cell line	Location
HPA041325	A-431	Endoplasmic reticulum
HPA041325	U-2 OS	Endoplasmic reticulum
HPA041325	U-251 MG	Endoplasmic reticulum
HPA043508	A-431	No staining
HPA043508	MCF7	No staining
HPA043508	U-2 OS	Endoplasmic reticulum

siRNA VALIDATION

Antibody

HPA041325

siRNA validation

Supported: Significant downregulation 10-25% by one/two siRNA:s

Analysis performed in

Cytoplasmic region of segmented cells in 10x-images

siRNA 1

RFI

79%

p-value

< 0.01


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Thumbnail 10x	Thumbnail 40x	Antibody	Sample	Cell line
		HPA041325	siRNA 1	U-2 OS
		HPA041325	scrambled	U-2 OS

MOUSE CELLS

Main location

Endoplasmic reticulum

Mouse gene

ENSMUSG00000001467 (version 83)


	Toggle channels	Low High G1 S G2 M N/A

Thumbnail	Antibody	Cell line	Location
	HPA043508	NIH 3T3	Endoplasmic reticulum

GENE INFORMATION
Gene name	CYP51A1 (HGNC Symbol)
Synonyms	CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1
Description	Cytochrome P450, family 51, subfamily A, polypeptide 1 (HGNC Symbol)
Entrez gene summary	This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
Chromosome	7
Cytoband	q21.2
Chromosome location (bp)	92112151 - 92142952
Protein evidence	Evidence at protein level (all genes)
Ensembl	ENSG00000001630 (version 83.38)
Entrez gene	1595
UniProt	Q16850 (UniProt - Evidence at protein level)
neXtProt	NX_Q16850
Antibodypedia	CYP51A1 antibodies

PROTEIN BROWSER

CYP51A1-001	CYP51A1-002	CYP51A1-005	CYP51A1-006

PROTEIN INFORMATION
Splice variant	UniProt	Protein class	Gene ontology	Length & mass	Signal peptide (predicted)	Transmembrane regions (predicted)
CYP51A1-001 ENSP00000003100 ENST00000003100	A0A0C4DFL7 [Direct mapping] Lanosterol 14-alpha demethylase Show all	Predicted membrane proteins Prediction method-based Membrane proteins predicted by MDM MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins Phobius predicted membrane proteins SCAMPI predicted membrane proteins SPOCTOPUS predicted membrane proteins THUMBUP predicted membrane proteins TMHMM predicted membrane proteins # TM segments-based 1TM proteins predicted by MDM Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005506 [iron ion binding] GO:0008398 [sterol 14-demethylase activity] GO:0016021 [integral component of membrane] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0020037 [heme binding] GO:0033488 [cholesterol biosynthetic process via 24,25-dihydrolanosterol] GO:0043231 [intracellular membrane-bounded organelle] GO:0055114 [oxidation-reduction process] Show all	509 aa 57.3 kDa	Yes	1
CYP51A1-002 ENSP00000406757 ENST00000450723	Q16850 [Direct mapping] Lanosterol 14-alpha demethylase Show all	Enzymes ENZYME proteins Oxidoreductases MEMSAT3 predicted membrane proteins MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins FDA approved drug targets Small molecule drugs Protein evidence (Kim et al 2014) Protein evidence (Ezkurdia et al 2014) Show all	GO:0005506 [iron ion binding] GO:0005783 [endoplasmic reticulum] GO:0005789 [endoplasmic reticulum membrane] GO:0006694 [steroid biosynthetic process] GO:0006695 [cholesterol biosynthetic process] GO:0006805 [xenobiotic metabolic process] GO:0008398 [sterol 14-demethylase activity] GO:0016020 [membrane] GO:0016021 [integral component of membrane] GO:0016125 [sterol metabolic process] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0020037 [heme binding] GO:0044281 [small molecule metabolic process] GO:0055114 [oxidation-reduction process] GO:0070988 [demethylation] Show all	404 aa 46.3 kDa	No	0
CYP51A1-005 ENSP00000412536 ENST00000435873	C9IYR8 [Direct mapping] Lanosterol 14-alpha demethylase Show all	Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all		30 aa 3.4 kDa	No	0
CYP51A1-006 ENSP00000394268 ENST00000422867	H7C0D0 [Direct mapping] Lanosterol 14-alpha demethylase Show all	MEMSAT-SVM predicted membrane proteins SPOCTOPUS predicted membrane proteins Predicted intracellular proteins Protein evidence (Ezkurdia et al 2014) Show all	GO:0004497 [monooxygenase activity] GO:0005506 [iron ion binding] GO:0016705 [oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen] GO:0020037 [heme binding] GO:0055114 [oxidation-reduction process] Show all	222 aa 25.5 kDa	No	0