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LAP3

ANTIBODY INFORMATION
 
 

Antibody HPA029606

  

Antibody HPA029607

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA029606   HPA029607  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 6   6  

References

    

Proper citation

 Atlas Antibodies Cat#HPA029606, RRID:AB_10599288   Atlas Antibodies Cat#HPA029607, RRID:AB_10611381  

VALIDATION SUMMARY
 
 

Antibody HPA029606

  

Antibody HPA029607

  
Immunocytochemistry

Supported    
Immunohistochemistry


Validated

Validated
  

Validated

Validated
  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA029606

  

Antibody HPA029607

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, cytosol & midbody.    

Antibody dilution

 1:7    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA029606

  

Antibody HPA029607

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 
 

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA029607 across 46 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA029606 across 46 cell lines.		  
 

Formal validation: Independent
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

Figure description

 Distribution of protein expression (antibody staining).
Spearman correlation with HPA029607 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA029606 across 74 cell types.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 51 cell types		   RNA: detected in 37 tissues
Protein: detected in 54 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:300  

Literature conformity

 Consistent with gene/protein characterization data.   Consistent with gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA029606

  

Antibody HPA029607

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 56.2   56.2, 52.8  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA029606

  

Antibody HPA029607

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA029606

  

Antibody HPA029607

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 92   86  

Antigen sequence

 
AGRVVVRRLAVRRFGSRSLSTADMTKGLVLGIYSKEKEDDVPQFTSAGEN
FDKLLAGKLRETLNISGPPLKAGKTRTFYGLHQDFPSVVLVG
  
QENWHEGKENIRAAVAAGCRQIQDLELSSVEVDPCGDAQAAAEGAVLGLY
EYDDLKQKKKMAVSAKLYGSGDQEAWQKGVLFASGQ
 

Matching transcripts

 LAP3-001 - ENSP00000226299 [100%]
LAP3-201 - ENSP00000481000 [100%]
  LAP3-001 - ENSP00000226299 [100%]
LAP3-010 - ENSP00000476028 [100%]
LAP3-201 - ENSP00000481000 [100%]
 

Other gene match

    
ANTIGEN VIEW
LAP3-001
LAP3-010
LAP3-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.