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Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.
Immunohistochemical staining of human pancreas shows membranous positivity in islets of Langerhans.
Expression
RNA: detected in 37 tissues Protein: detected in 26 cell types
RNA: detected in 37 tissues Protein: detected in 41 cell types
Retrieval
HIER pH6
HIER pH9
Antibody dilution
1:125
1:100
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA035304
Antibody CAB000020
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400863)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
18.8, 17.1
20.6, 18.8, 17.7, 17.1, 16, 8
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA035304
Antibody CAB000020
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
