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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
Antibody dilution
1:24
1:125
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA053660
Antibody CAB005884
Standard validation
Supported
Figure description
Immunohistochemical staining of human placenta shows distinct nuclear positivity in trophoblastic cells.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
Antibody dilution
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA053660
Antibody CAB005884
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
95.2, 92.9
95.2, 92.9, 32.5, 21.3
Antibody dilution
1:120
1:500
PROTEIN ARRAY
Antibody HPA053660
Antibody CAB005884
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
