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POLR2J

ANTIBODY INFORMATION
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA042997   HPA044010   HPA046970  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 POLR2J3 - ENSG00000168255 [100%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J3 - ENSG00000168255 [100%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J3 - ENSG00000168255 [100%]
POLR2J2 - ENSG00000228049 [97%]
RP11-514P8.7 - ENSG00000270249 [97%]
  

Released in version

 11   11   11  

References

      

Proper citation

 Atlas Antibodies Cat#HPA042997, RRID:AB_2678262   Atlas Antibodies Cat#HPA044010, RRID:AB_2678773   Atlas Antibodies Cat#HPA046970, RRID:AB_2679882  

VALIDATION SUMMARY
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  
Immunocytochemistry

    Approved  
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

     Approved  

     

Figure description

     Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

     1:43  

Literature conformity

     The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human salivary gland shows strong cytoplasmic positivity in red blood cells.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in bile duct cells.   Immunohistochemical staining of human liver shows moderate cytoplasmic and nuclear positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 3 cell types		   RNA: detected in 37 tissues
Protein: detected in 15 cell types		   RNA: detected in 37 tissues
Protein: detected in 28 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:25   1:75   1:120  

Literature conformity

 Not consistent with gene/protein characterization data.   Not consistent with gene/protein characterization data.   Partly consistent with gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.   Mainly not consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 17.2, 17.1   17.2, 17.1, 13.3, 13.1   30.9, 30.4, 17.2, 17.1, 13.3, 13.1, 8.4, 8  

Antibody dilution

 1:250   1:250   1:210  

PROTEIN ARRAY
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:1700  

ANTIGEN INFORMATION
 
 

Antibody HPA042997

  

Antibody HPA044010

  

Antibody HPA046970

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 43   54   31  

Antigen sequence

 
VGWTLARVPRPGTALACFFGGPQGEAAVMEEQGLPPQAPGHVD
  
DPQVLFAGYKVPHPLEHKIIIRVQTTPDYSPQEAFTNAITDLISELSLLE
ERFR
  
KITINKDTKVPNACLFTINKEDHTLGNIIKS
 

Matching transcripts

 POLR2J-201 - ENSP00000377383 [98%]
  POLR2J-001 - ENSP00000292614 [100%]
POLR2J-201 - ENSP00000377383 [100%]
  POLR2J-001 - ENSP00000292614 [100%]
POLR2J-201 - ENSP00000377383 [100%]
 

Other gene match

 POLR2J3 - ENSG00000168255 [100%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J3 - ENSG00000168255 [100%]
POLR2J2 - ENSG00000228049 [100%]
   POLR2J3 - ENSG00000168255 [100%]
POLR2J2 - ENSG00000228049 [97%]
RP11-514P8.7 - ENSG00000270249 [97%]
  
ANTIGEN VIEW
POLR2J-001
POLR2J-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.